LOCUS       sequence-228991-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(844..861)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gggcggagaa atctccctga gaagcgcaga gaagggatcc agcggcggcg gcagcggcgg
       61 cggcggcagc actgagtaca aactggtggt ggttggagca ggtggtgttg ggaaaagcgc
      121 actgacaatc cagctaatcc agaaccactt tgtagatgaa tatgatccca ccatagagga
      181 ttctgccaga aaacaagtgg ttatagatgg tgaaacctgt ttgttggaca tactggatac
      241 agctggaaaa gaagagtaca gtgccatgag agaccaatac atgaggacag gcgaaggctt
      301 cctctgtgta tttgccatca ataatagcaa gtcatttgcg gatattaacc tctacaggga
      361 gcagattaag cgagtaaaag actcggatga tgtacctatg gtgctagtgg gaaacaagtg
      421 tgatttgcca acaaggacag ttgatacaaa acaagcccac gaactggcca agagttacgg
      481 gattccattc attgaaacct cagccaagac cagacagggt gttgaagatg ctttttacac
      541 actggtaaga gaaatacgcc agtaccgaat gaaaaaactc aacagcagtg atgatgggac
      601 tcagggttgt atgggattgc catgtgtggt gatgtaactc gagtctagag ggcccgacgc
      661 gtctggccac cggtcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
      721 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
      781 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
      841 cccctctcgc caaaggaatg c
//
