LOCUS       sequence-228997-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-N-Ras wildtype fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-NRAS-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..45
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             116..139
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             146..172
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             182..199
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
       61 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta ccatggatta
      121 taaagatgac gacgataaag caggttaccc atacgatgtg cctgactatg ctgcaggttc
      181 acatcatcac caccatcatg gatccatgac tgagtacaaa ctggtggtgg ttggagcagg
      241 tggtgttggg aaaagcgcac tgacaatcca gctaatccag aaccactttg tagatgaata
      301 tgatcccacc atagaggatt cttacagaaa acaagtggtt atagatggtg aaacctgttt
      361 gttggacata ctggatacag ctggacaaga agagtacagt gccatgagag accaatacat
      421 gaggacaggc gaaggcttcc tctgtgtatt tgccatcaat aatagcaagt catttgcgga
      481 tattaacctc tacagggagc agattaagcg agtaaaagac tcggatgatg tacctatggt
      541 gctagtggga aacaagtgtg atttgccaac aaggacagtt gatacaaaac aagcccacga
      601 actggccaag agttacggga ttccattcat tgaaacctca gccaagacca gacagggtgt
      661 tgaagatgct ttttacacac tggtaagaga aatacgccag taccgaatga aaaaactcaa
      721 cagcagtgat gatgggactc agggttgtat gggattgcca tgtgtggtga tgtaagtcga
      781 gtctagaggg cccgacgcgt ctggccaccg gtcgcccctc tccctccccc ccccctaacg
      841 ttac
//
