LOCUS       sequence-228999-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-NRASQ61K-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..41
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             142..168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             178..195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
       61 gctgttttga cctccataga agacaccgac tctactagag gatctaccat ggattataaa
      121 gatgacgacg ataaagcagg ttacccatac gatgtgcctg actatgctgc aggttcacat
      181 catcaccacc atcatggatc catgactgag tacaaactgg tggtggttgg agcaggtggt
      241 gttgggaaaa gcgcactgac aatccagcta atccagaacc actttgtaga tgaatatgat
      301 cccaccatag aggattctta cagaaaacaa gtggttatag atggtgaaac ctgtttgttg
      361 gacatactgg atacagctgg aaaagaagag tacagtgcca tgagagacca atacatgagg
      421 acaggcgaag gcttcctctg tgtatttgcc atcaataata gcaagtcatt tgcggatatt
      481 aacctctaca gggagcagat taagcgagta aaagactcgg atgatgtacc tatggtgcta
      541 gtgggaaaca agtgtgattt gccaacaagg acagttgata caaaacaagc ccacgaactg
      601 gccaagagtt acgggattcc attcattgaa acctcagcca agaccagaca gggtgttgaa
      661 gatgcttttt acacactggt aagagaaata cgccagtacc gaatgaaaaa actcaacagc
      721 agtgatgatg ggactcaggg ttgtatggga ttgccatgtg tggtgatgta agtcgagtct
      781 agagggcccg acgcgtctgg ccaccggtcg cccctctccc tccccccccc ctaacgttac
      841 tggccgaagc cgct
//
