LOCUS       sequence-22900-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  modified pENTR vector for N-terminal STREP tag fusion with your gene
            of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE1n
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             135..158
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(229..328)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(440..459)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(518..537)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctacngggcc aatatggatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcantg cttttttata atgccaactt tgtacaaaaa agcaggcttt aaaggaacca
      121 attcagtcga catgtggagc catccgcagt tcgaaaaagg cggcagcggc ggatccggta
      181 cagatttcgg acatgcggcc gcataagtag ctgactcgag atatctagac ccagctttct
      241 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      301 ctatcagtca aaataaaatc attatttgcn ntccgggtgc anntctggcc cgtgtctcaa
      361 aatctctgan gttacattgc acnagataaa aatatatcat catgaacaat aaaactgtct
      421 gcttacataa acagnaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
      481 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
      541 gtcgggcaat caggtgcgac antctatcgc ntgnatggga agcccgatgc gccagagttg
      601 tttctgaaac atggcanang tngcgttgcc cnatgntgtt accgat
//
