LOCUS       sequence-229000-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-NRASQ61K-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..64
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             71..97
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctccatagaa gacaccgact ctactagagg atctaccatg gattataaag atgacgacga
       61 taaagcaggt tacccatacg atgtgcctga ctatgctgca ggttcacatc atcaccacca
      121 tcatggatcc atgactgagt acaaactggt ggtggttgga gcaggtggtg ttgggaaaag
      181 cgcactgaca atccagctaa tccagaacca ctttgtagat gaatatgatc ccaccataga
      241 ggattcttac agaaaacaag tggttataga tggtgaaacc tgtttgttgg acatactgga
      301 tacagctgga aaagaagagt acagtgccat gagagaccaa tacatgagga caggcgaagg
      361 cttcctctgt gtatttgcca tcaataatag caagtcattt gcggatatta acctctacag
      421 ggagcagatt aagcgagtaa aagactcgga tgatgtacct atggtgctag tgggaaacaa
      481 gtgtgatttg ccaacaagga cagttgatac aaaacaagcc cacgaactgg ccaagagtta
      541 cgggattcca ttcattgaaa cctcagccaa gaccagacag ggtgttgaag atgcttttta
      601 cacactggta agagaaatac gccagtaccg aatgaaaaaa ctcaacagca gtgatgatgg
      661 gactcagggt tgtatgggat tgccatgtgt ggtgatgtaa gtcgagtcta gagggcccga
      721 cgcgtctggc caccggtcgc c
//
