LOCUS       sequence-229003-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-NRASQ61K/T35A-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..41
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             48..74
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             84..101
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctagaggatc taccatggat tataaagatg acgacgataa agcaggttac ccatacgatg
       61 tgcctgacta tgctgcaggt tcacatcatc accaccatca tggatccact gagtacaaac
      121 tggtggtggt tggagcaggt ggtgttggga aaagcgcact gacaatccag ctaatccaga
      181 accactttgt agatgaatat gatcccgcca tagaggattc ttacagaaaa caagtggtta
      241 tagatggtga aacctgtttg ttggacatac tggatacagc tggaaaagaa gagtacagtg
      301 ccatgagaga ccaatacatg aggacaggcg aaggcttcct ctgtgtattt gccatcaata
      361 atagcaagtc atttgcggat attaacctct acagggagca gattaagcga gtaaaagact
      421 cggatgatgt acctatggtg ctagtgggaa acaagtgtga tttgccaaca aggacagttg
      481 atacaaaaca agcccacgaa ctggccaaga gttacgggat tccattcatt gaaacctcag
      541 ccaagaccag acagggtgtt gaagatgctt tttacacact ggtaagagaa atacgccagt
      601 accgaatgaa aaaactcaac agcagtgatg atgggactca gggttgtatg ggattgccat
      661 gtgtggtgat gtaactcgag tctagagggc c
//
