LOCUS       sequence-229008-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-NRASQ61K/Y40A-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..44
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             115..138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             145..171
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             181..198
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
       61 cacgctgttt tgacctccat agaagacacc gactctacta gaggatctac catggattat
      121 aaagatgacg acgataaagc aggttaccca tacgatgtgc ctgactatgc tgcaggttca
      181 catcatcacc accatcatgg atccactgag tacaaactgg tggtggttgg agcaggtggt
      241 gttgggaaaa gcgcactgac aatccagcta atccagaacc actttgtaga tgaatatgat
      301 cccaccatag aggattctgc cagaaaacaa gtggttatag atggtgaaac ctgtttgttg
      361 gacatactgg atacagctgg aaaagaagag tacagtgcca tgagagacca atacatgagg
      421 acaggcgaag gcttcctctg tgtatttgcc atcaataata gcaagtcatt tgcggatatt
      481 aacctctaca gggagcagat taagcgagta aaagactcgg atgatgtacc tatggtgcta
      541 gtgggaaaca agtgtgattt gccaacaagg acagttgata caaaacaagc ccacgaactg
      601 gccaagagtt acgggattcc attcattgaa acctcagcca agaccagaca gggtgttgaa
      661 gatgcttttt acacactggt aagagaaata cgccagtacc gaatgaaaaa actcaacagc
      721 agtgatgatg ggactcaggg ttgtatggga ttgccatgtg tggtgatgta actcgagtct
      781 agagggcccg acgcgtctgg ccaccggtcg cccctctccc tccccccccc ctaacgttac
      841 tggccgaagc cgcttggaat a
//
