LOCUS       sequence-229009-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-NRASQ61K/Y40A-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..40
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             132..155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             162..188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             198..215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga nccgtcagat
       61 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctactagag
      121 gatctaccat ggattataaa gatgacgacg ataaagcagg ttacccatac gatgtgcctg
      181 actatgctgc aggttcacat catcaccacc atcatggatc cactgagtac aaactggtgg
      241 tggttggagc aggtggtgtt gggaaaagcg cactgacaat ccagctaatc cagaaccact
      301 ttgtagatga atatgatccc accatagagg attctgccag aaaacaagtg gttatagatg
      361 gtgaaacctg tttgttggac atactggata cagctggaaa agaagagtac agtgccatga
      421 gagaccaata catgaggaca ggcgaaggct tcctctgtgt atttgccatc aataatagca
      481 agtcatttgc ggatattaac ctctacaggg agcagattaa gcgagtaaaa gactcggatg
      541 atgtacctat ggtgctagtg ggaaacaagt gtgatttgcc aacaaggaca gttgatacaa
      601 aacaagccca cgaactggcc aagagttacg ggattccatt cattgaaacc tcagccaaga
      661 ccagacaggg tgttgaagat gctttttaca cactggtaag agaaatacgc cagtaccgaa
      721 tgaaaaaact caacagcagt gatgatggga ctcagggttg tatgggattg ccatgtgtgg
      781 tgatgtaact cgagtctaga gggcccgacg cgtctggcca ccggtcgccc ctctccctcc
      841 ccc
//
