LOCUS       sequence-22901-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, 
            Greenblatt JF, Dilworth FJ, Rudnicki MA
  TITLE     Pax7 activates myogenic genes by recruitment of a histone 
            methyltransferase complex.
  JOURNAL   Nat Cell Biol. 2008 Jan . 10(1):77-84.
  PUBMED    18066051
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Jan . 10(1):77-84."
COMMENT     Sequence Label: pBRIT HA/FLAG
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..55
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             128..154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             165..236
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    254..287
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(299..319)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        304..633
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      484..619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(643..662)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 acttcgtata tgtatgctat acgaagttat ggatctaatt aaccctcact aaagggaaca
       61 aaagctggag ctccaccgcg gtggcggccg ctctagcccg ggcggatccg aattcgcatg
      121 cgtcgactat ccatacgacg tgcctgacta cgcctgaact cgaggactac aaggatgacg
      181 atgacaagga ttacaaagac gacgatgata aggactataa ggatgatgac gacaaataat
      241 agtctagaga tccataactt cgtataatgt atgctatacg aagttatctc gaccctgtgg
      301 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      361 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
      421 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
      481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
      541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
      601 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc
      661 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgc
//
