LOCUS       sequence-229012-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-N-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FLAG-NRAS-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..43
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     40..64
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             132..155
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
       61 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctacta
      121 gaggatccat ggattataaa gatgacgacg ataaaactga gtacaaactg gtggtggttg
      181 gagcaggtgg tgttgggaaa agcgcactga caatccagct aatccagaac cactttgtag
      241 atgaatatga tcccaccata gaggattctt acagaaaaca agtggttata gatggtgaaa
      301 cctgtttgtt ggacatactg gatacagctg gacaagaaga gtacagtgcc atgagagacc
      361 aatacatgag gacaggcgaa ggcttcctct gtgtatttgc catcaataat agcaagtcat
      421 ttgcggatat taacctctac agggagcaga ttaagcgagt aaaagactcg gatgatgtac
      481 ctatggtgct agtgggaaac aagtgtgatt tgccaacaag gacagttgat acaaaacaag
      541 cccacgaact ggccaagagt tacgggattc cattcattga aacctcagcc aagaccagac
      601 agggtgttga agatgctttt tacacactgg taagagaaat acgccagtac cgaatgaaaa
      661 aactcaacag cagtgatgat gggactcagg gttgtatggg attgccatgt gtggtgatgt
      721 aactcgagtc tagagggccc gacgcgtctg gccaccggtc gcccctctcc ctcccccccc
      781 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
      841 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacct
//
