LOCUS       sequence-229013-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-N-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FLAG-NRAS-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..60
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11..31
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     57..81
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             149..172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
       61 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      121 agacaccgac tctactagag gatccatgga ttataaagat gacgacgata aaactgagta
      181 caaactggtg gtggttggag caggtggtgt tgggaaaagc gcactgacaa tccagctaat
      241 ccagaaccac tttgtagatg aatatgatcc caccatagag gattcttaca gaaaacaagt
      301 ggttatagat ggtgaaacct gtttgttgga catactggat acagctggac aagaagagta
      361 cagtgccatg agagaccaat acatgaggac aggcgaaggc ttcctctgtg tatttgccat
      421 caataatagc aagtcatttg cggatattaa cctctacagg gagcagatta agcgagtaaa
      481 agactcggat gatgtaccta tggtgctagt gggaaacaag tgtgatttgc caacaaggac
      541 agttgataca aaacaagccc acgaactggc caagagttac gggattccat tcattgaaac
      601 ctcagccaag accagacagg gtgttgaaga tgctttttac acactggtaa gagaaatacg
      661 ccagtaccga atgaaaaaac tcaacagcag tgatgatggg actcagggtt gtatgggatt
      721 gccatgtgtg gtgatgtaac tcgagtctag agggcccgac gcgtctggcc accggtcg
//
