LOCUS       sequence-229016-         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-N-Ras Q61K fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FLAG-NRASQ61K-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagaggat ccatggatta taaagatgac gacgataaaa ctgagtacaa actggtggtg
       61 gttggagcag gtggtgttgg gaaaagcgca ctgacaatcc agctaatcca gaaccacttt
      121 gtagatgaat atgatcccac catagaggat tcttacagaa aacaagtggt tatagatggt
      181 gaaacctgtt tgttggacat actggataca gctggaaaag aagagtacag tgccatgaga
      241 gaccaataca tgaggacagg cgaaggcttc ctctgtgtat ttgccatcaa taatagcaag
      301 tcatttgcgg atattaacct ctacagggag cagattaagc gagtaaaaga ctcggatgat
      361 gtacctatgg tgctagtggg aaacaagtgt gatttgccaa caaggacagt tgatacaaaa
      421 caagcccacg aactggccaa gagttacggg attccattca ttgaaacctc agccaagacc
      481 agacagggtg ttgaagatgc tttttacaca ctggtaagag aaatacgcca gtaccgaatg
      541 aaaaaactca acagcagtga tgatgggact cagggttgta tgggattgcc atgtgtggtg
      601 atgtaactcg agtctagagg gcccgacgcg tctggccacc ggtcg
//
