LOCUS       sequence-229017-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-N-Ras Q61K fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FLAG-NRASQ61K-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..44
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
       61 cacgctgttt tgacctccat agaagacacc gactctacta gaggatccat ggattataaa
      121 gatgacgacg ataaaactga gtacaaactg gtggtggttg gagcaggtgg tgttgggaaa
      181 agcgcactga caatccagct aatccagaac cactttgtag atgaatatga tcccaccata
      241 gaggattctt acagaaaaca agtggttata gatggtgaaa cctgtttgtt ggacatactg
      301 gatacagctg gaaaagaaga gtacagtgcc atgagagacc aatacatgag gacaggcgaa
      361 ggcttcctct gtgtatttgc catcaataat agcaagtcat ttgcggatat taacctctac
      421 agggagcaga ttaagcgagt aaaagactcg gatgatgtac ctatggtgct agtgggaaac
      481 aagtgtgatt tgccaacaag gacagttgat acaaaacaag cccacgaact ggccaagagt
      541 tacgggattc cattcattga aacctcagcc aagaccagac agggtgttga agatgctttt
      601 tacacactgg taagagaaat acgccagtac cgaatgaaaa aactcaacag cagtgatgat
      661 gggactcagg gttgtatggg attgccatgt gtggtgatgt aactcgagtc tagagggccc
      721 gacgcgtctg gccaccggtc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
      781 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
      841 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgac
//
