LOCUS       sequence-22902-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p406GAL1
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     409..432
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     453..469
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     453..469
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(503..519)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(504..520)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(538..556)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ttagaagccg ccgagcgggt gacagccctc cgaaggaaga ctctcctccg tgcgtcctcg
       61 tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat
      121 aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca gtaacctggc
      181 cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa tgcgattagt
      241 tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga tctattaaca
      301 gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca ttttcggttt
      361 gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa tatacctcta
      421 tactttaacg tcaaggagaa aaaaccccgg attctagaac tagtggatcc cccgggctgc
      481 aggaattcga tatcaagctt atcgataccg tcgacctcga gtcatgtaat tagttatgtc
      541 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
      601 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
      661 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
      721 g
//
