LOCUS       sequence-229020-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-RHEB fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FLAG-RHEB-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..45
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             113..136
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
       61 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcca tggattataa
      121 agatgacgac gataaacctc agtcaaaatc tagaaagatt gctattttgg ggtatagatc
      181 agtcgggaag agctccctta ctatacaatt tgtcgaaggg caattcgttg atagttacga
      241 ccctaccatc gagaacacct tcaccaaact catcaccgtt aatggtcagg aatatcatct
      301 tcaactcgtg gatacggctg ggcaagacga atattcaatt ttcccccaga cttatagcat
      361 cgatattaac ggatatattc tggtttattc cgttaccagt atcaagagct tcgaggtcat
      421 taaggtcatc catggaaaat tgttggatat ggtcggcaaa gtacagattc ccataatgtt
      481 ggtcggcaac aaaaaggatt tgcacatgga gagagtgatt tcctatgagg aggggaaagc
      541 tcttgcagaa tcctggaatg ctgccttcct tgagtcctct gcgaaggaga accaaacagc
      601 agtagacgta tttaggagga ttatcctgga agccgagaaa atggatggtg ccgcgtcaca
      661 agggaaaagt agttgtagtg ttatgtgact cgagtctaga gggcccgacg cgtctggcca
      721 ccggtcgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      781 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      841 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      901 ccaa
//
