LOCUS       sequence-229021-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-RHEB fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FLAG-RHEB-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..35
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaggatccat ggattataaa gatgacgacg ataaacctca gtcaaaatct agaaagattg
       61 ctattttggg gtatagatca gtcgggaaga gctcccttac tatacaattt gtcgaagggc
      121 aattcgttga tagttacgac cctaccatcg agaacacctt caccaaactc atcaccgtta
      181 atggtcagga atatcatctt caactcgtgg atacggctgg gcaagacgaa tattcaattt
      241 tcccccagac ttatagcatc gatattaacg gatatattct ggtttattcc gttaccagta
      301 tcaagagctt cgaggtcatt aaggtcatcc atggaaaatt gttggatatg gtcggcaaag
      361 tacagattcc cataatgttg gtcggcaaca aaaaggattt gcacatggag agagtgattt
      421 cctatgagga ggggaaagct cttgcagaat cctggaatgc tgccttcctt gagtcctctg
      481 cgaaggagaa ccaaacagca gtagacgtat ttaggaggat tatcctggaa gccgagaaaa
      541 tggatggtgc cgcgtcacaa gggaaaagta gttgtagtgt tatgtgactc gagtctagag
      601 ggcccgacgc gtctg
//
