LOCUS       sequence-229029-       10410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RMCE donor vector for inducible expression of SOX10 constitutive 
            expression of m2rtTA (generated from plasmid #112668).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: pZ M2rtTA_CAGG TetON-Sox10
FEATURES             Location/Qualifiers
     source          1..10410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        209..247
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     244..268
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1702..1721)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1746..1793
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    2048..2159
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2313..2912)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2396..2416)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2893..2912
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(2922..2975)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    2996..3021
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(3025..3058)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    3065..3868
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(3882..3901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3883..3901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3911..3928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3911..3927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3920..3942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4069..4524
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4156..4175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4366..4387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4550..4654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4655..4723,4724..5515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4873..4892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5686..6274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6175..6194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6428..6445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6562..6583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6598..6603,6604..6621,6622..6628)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6636..6652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6641..6663
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6660..6676
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6660..6676
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6695..6715
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6697..6715
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    6745..7581
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(7591..7624)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    7631..7855
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(7869..7877,7878..7913,7914..7916,7917..7952,
                     7953..7955,7956..7991,7992..7997,7998..8615))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(8643..8662)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          8670..9686
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(8724..8746)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(9687..9962)
                     /label=chicken beta-actin promoter
     enhancer        9967..10350
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
       61 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      121 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
      181 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
      241 cagagctcgt ttagtgaacc gtcagatcgc ttaaggccac catggcggag gagcaggacc
      301 tatcggaggt ggagctgagc cccgtgggct cggaggagcc ccgctgcctg tccccgggga
      361 gcgcgccctc gctagggccc gacggcggcg gcggcggatc gggcctgcga gccagcccgg
      421 ggccaggcga gctgggcaag gtcaagaagg agcagcagga cggcgaggcg gacgatgaca
      481 agttccccgt gtgcatccgc gaggccgtca gccaggtgct cagcggctac gactggacgc
      541 tggtgcccat gcccgtgcgc gtcaacggcg ccagcaaaag caagccgcac gtcaagcggc
      601 ccatgaacgc cttcatggtg tgggctcagg cagcgcgcag gaagctcgcg gaccagtacc
      661 cgcacctgca caacgctgag ctcagcaaga cgctaggcaa gctctggagg ctgctgaacg
      721 aaagtgacaa gcgccccttc atcgaggagg ctgagcggct ccgtatgcag cacaagaaag
      781 accacccgga ctacaagtac cagcccaggc ggcggaagaa cgggaaggcc gcccagggcg
      841 aggcggagtg ccccggtggg gaggccgagc aaggtgggac cgccgccatc caggcccact
      901 acaagagcgc ccacttggac caccggcacc caggagaggg ctcccccatg tcagatggga
      961 accccgagca cccctcaggc cagagccatg gcccacccac ccctccaacc accccgaaga
     1021 cagagctgca gtcgggcaag gcagacccga agcgggacgg gcgctccatg ggggagggcg
     1081 ggaagcctca catcgacttc ggcaacgtgg acattggtga gatcagccac gaggtaatgt
     1141 ccaacatgga gacctttgat gtggctgagt tggaccagta cctgccgccc aatgggcacc
     1201 caggccacgt gagcagctac tcagcagccg gctatgggct gggcagtgcc ctggccgtgg
     1261 ccagtggaca ctccgcctgg atctccaagc caccaggcgt ggctctgccc acggtctcac
     1321 cacctggtgt ggatgccaaa gcccaggtga agacagagac cgcggggccc caggggcccc
     1381 cacactacac cgaccagcca tccacctcac agatcgccta cacctccctc agcctgcccc
     1441 actatggctc agccttcccc tccatctccc gcccccagtt tgactactct gaccatcagc
     1501 cctcaggacc ctattatggc cactcgggcc aggcctctgg cctctactcg gccttctcct
     1561 atatggggcc ctcgcagcgg cccctctaca cggccatctc tgaccccagc ccctcagggc
     1621 cccagtccca cagccccaca cactgggagc agccagtata tacgacactg tcccggccct
     1681 aaacgcgtgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
     1741 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg
     1801 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca
     1861 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg
     1921 gctcgcagcc aacgtcgggg cggcaggccc tgccatagct cgagtgctat tgtcttccca
     1981 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     2041 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     2101 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
     2161 cgaggctgat cagcgggttt aaacgggccc tctagagtcg gtgggcctcg ggggcgggtg
     2221 cggggtcggc ggggccgccc cgggtggctt cggtcggagc catggggtcg tgcgctcctt
     2281 tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac
     2341 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     2401 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     2461 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     2521 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     2581 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     2641 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     2701 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     2761 tcttgcagct cggtgacccg ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg
     2821 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg
     2881 gcgaggcgca ccgtgggctt gtactcggtc atttcgagcc tagggccggg attctcctcc
     2941 acgtcaccgc atgttagaag acttcctctg ccctctccgc tgccagatct gaggccctgt
     3001 gggaggaaga gaagaggtca ggccgaagtt cctatactat ttgaagaata ggaacttcaa
     3061 gcttgcccca ctgtggggtg gaggggacag ataaaagtac ccagaaccag agccacatta
     3121 accggccctg ggaatataag gtggtcccag ctcggggaca caggatccct ggaggcagca
     3181 aacatgctgt cctgaagtgg acataggggc ccgggttgga ggaagaagac tagctgagct
     3241 ctcggacccc tggaagatgc catgacaggg ggctggaaga gctagcacag actagagagg
     3301 taaggggggt aggggagctg cccaaatgaa aggagtgaga ggtgacccga atccacagga
     3361 gaacggggtg tccaggcaaa gaaagcaaga ggatggagag gtggctaaag ccagggagac
     3421 ggggtacttt ggggttgtcc agaaaaacgg tgatgatgca ggcctacaag aaggggaggc
     3481 gggacgcaag ggagacatcc gtcggagaag gccatcctaa gaaacgagag atggcacagg
     3541 ccccagaagg agaaggaaaa gggaacccag cgagtgaaga cggcatgggg ttgggtgagg
     3601 gaggagagat gcccggagag gacccagaca cggggaggat ccgctcagag gacatcacgt
     3661 ggtgcagcgc cgagaaggaa gtgctccgga aagagcatcc ttgggcagca acacagcaga
     3721 gagcaagggg aagagggagt ggaggaagac ggaacctgaa ggaggcggca gggaaggatc
     3781 tgggccagcc gtagaggtga cccaggccac aagctgcaga cagaaagcgg cacaggccca
     3841 ggggagagaa tgctggtcag agaaagcagg tacccaattc gccctatagt gagtcgtatt
     3901 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3961 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4021 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     4081 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4141 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4201 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4261 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4321 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4381 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4441 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4501 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4561 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4621 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4681 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4741 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4801 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4861 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4921 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4981 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5041 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5101 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5161 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5221 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5281 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5341 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5401 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5461 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5521 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5581 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5821 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6301 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6361 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6421 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6481 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6541 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6601 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6661 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6721 aaagctggag ctccaccgcg gtggagagca gagccaggaa cccctgtagg gaaggggcag
     6781 gagagccagg ggcatgagat ggtggacgag gaagggggac agggaagcct gagcgcctct
     6841 cctgggcttg ccaaggactc aaacccagaa gcccagagca gggccttagg gaagcgggac
     6901 cctgctctgg gcggaggaat atgtcccaga tagcactggg gactctttaa ggaaagaagg
     6961 atggagaaag agaaagggag tagaggcggc cacgacctgg tgaacaccta ggacgcacca
     7021 ttctcacaaa gggagttttc cacacggaca cccccctcct caccacagcc ctgccaggac
     7081 ggggctggct actggcctta tctcacaggt aaaactgacg cacggaggaa caatataaat
     7141 tggggactag aaaggtgaag agccaaagtt agaactcagg accaacttat tctgattttg
     7201 tttttccaaa ctgcttctcc tcttgggaag tgtaaggaag ctgcagcacc aggatcagtg
     7261 aaacgcacca gacagccgcg tcagagcagc tcaggttctg ggagagggta gcgcagggtg
     7321 gccactgaga accgggcagg tcacgcatcc cccccttccc tcccaccccc tgccaagctc
     7381 tccctcccag gatcctctct ggctccatcg taagcaaacc ttagaggttc tggcaaggag
     7441 agagatggct ccaggaaatg ggggtgtgtc accagataag gaatctgcct aacaggaggt
     7501 gggggttaga cccaatatca ggagactagg aaggaggagg cctaaggatg gggcttttct
     7561 gtcaccaatc ctgtccctag tagcggccgc gaagttccta tactttctag agaataggaa
     7621 cttcttcgaa ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
     7681 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
     7741 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
     7801 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
     7861 gcactagttt acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc
     7921 atatcaaggt caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca
     7981 agggcgtcgg ccggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt
     8041 agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct
     8101 tgtctcagaa gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact
     8161 tgatgctcct gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg
     8221 tacagtgcgt tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg
     8281 gtttcgtact gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt
     8341 gagaggagag cacagcggaa tgacttggcg ttgttccgca gaaagtcttg ccatgactcg
     8401 ccttccaggg ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg
     8461 agcagggccc gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt
     8521 tgagcgagtt tccttgtcgt caggccttcg ataccgactc cattgagtaa ttccagagcg
     8581 ccgtttatga ctttgctctt gtccagtcta gacatgaatt ctttgccaaa atgatgagac
     8641 agcacaataa ccagcacgtt gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg
     8701 ttagcagagg ctctagagcc gccggtcaca cgccagaagc cgaaccccgc cctgccccgt
     8761 cccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg gagaagggga
     8821 cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac
     8881 cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt cggctccgca
     8941 cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa aaggcaatgg
     9001 ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg ggccgccgcg cccctccccc
     9061 gagccctccc cggcccgagg cggccccgcc ccgcccggca cccccacctg ccgccacccc
     9121 ccgcccggca cggcgagccc cgcgccacgc cccgtacgga gccccgcacc cgaagccggg
     9181 ccgtgctcag caactcgggg aggggggtgc agggggggtt gcagcccgac cgacgcgccc
     9241 acaccccctg ctcacccccc cacgcacaca ccccgcacgc agcctttgtt cccctcgcag
     9301 ccccccccgc accgcggggc accgcccccg gccgcgctcc cctcgcgcac actgcggagc
     9361 gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc cgggcagcgc ggagccgcac
     9421 gcggcgctcc ccacgcacac acacacgcac gcaccccccg agccgctccc cccgcacaaa
     9481 gggccctccc ggagcccctc aaggctttca cgcagccaca gaaaagaaac aagccgtcat
     9541 taaaccaagc gctaattaca gcccggagga gaagggccgt cccgcccgct cacctgtggg
     9601 agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg cggcggcgga gcggggcacg
     9661 gggcgaaggc agcgcgcagc gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc
     9721 cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg attggctgcc
     9781 gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg
     9841 cgcccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa
     9901 aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc
     9961 gaccatcatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
    10021 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
    10081 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
    10141 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
    10201 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
    10261 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
    10321 ttaataacta gctagtcaat aatcaatgtc gacgatgatg ctgtacactc gaaacttttt
    10381 tcgaaggtcc gaggttctag acgagtttac
//
