LOCUS       sequence-229030-       11225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RMCE donor vector for inducible expression of SOX10 and GFP and 
            constitutive expression of m2rtTA (generated from plasmid #112668).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: pZ M2rtTA_CAGG TetON-Sox10 with GFP
FEATURES             Location/Qualifiers
     source          1..11225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    36..54
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    72..90
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    108..126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        209..247
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     244..268
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1707..1763
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1776..1778,1779..1781,1782..2495)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1821..1842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2082..2101)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2429..2450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2515..2534)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2559..2606
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    2861..2972
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3126..3725)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3209..3229)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3706..3725
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(3735..3788)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    3809..3834
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    complement(3838..3871)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    3878..4681
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(4695..4714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4696..4714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4724..4741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4724..4740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4733..4755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4882..5337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4969..4988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5179..5200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5363..5467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5468..5536,5537..6328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5686..5705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6499..7087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6988..7007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7241..7258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7375..7396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7411..7416,7417..7434,7435..7441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7449..7465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7454..7476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7473..7489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7473..7489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7508..7528
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7510..7528
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7558..8394
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    complement(8404..8437)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    8444..8668
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(8682..8690,8691..8726,8727..8729,8730..8765,
                     8766..8768,8769..8804,8805..8810,8811..9428))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9456..9475)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          9483..10499
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(9537..9559)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(10500..10777)
                     /label=chicken beta-actin promoter
     enhancer        10782..11165
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
       61 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
      121 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
      181 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
      241 cagagctcgt ttagtgaacc gtcagatcgc ttaaggccac catggcggag gagcaggacc
      301 tatcggaggt ggagctgagc cccgtgggct cggaggagcc ccgctgcctg tccccgggga
      361 gcgcgccctc gctagggccc gacggcggcg gcggcggatc gggcctgcga gccagcccgg
      421 ggccaggcga gctgggcaag gtcaagaagg agcagcagga cggcgaggcg gacgatgaca
      481 agttccccgt gtgcatccgc gaggccgtca gccaggtgct cagcggctac gactggacgc
      541 tggtgcccat gcccgtgcgc gtcaacggcg ccagcaaaag caagccgcac gtcaagcggc
      601 ccatgaacgc cttcatggtg tgggctcagg cagcgcgcag gaagctcgcg gaccagtacc
      661 cgcacctgca caacgctgag ctcagcaaga cgctgggcaa gctctggagg ctgctgaacg
      721 aaagtgacaa gcgccccttc atcgaggagg ctgagcggct ccgtatgcag cacaagaaag
      781 accacccgga ctacaagtac cagcccaggc ggcggaagaa cgggaaggcc gcccagggcg
      841 aggcggagtg ccccggtggg gaggccgagc aaggtgggac cgccgccatc caggcccact
      901 acaagagcgc ccacttggac caccggcacc caggagaggg ctcccccatg tcagatggga
      961 accccgagca cccctcaggc cagagccatg gcccacccac ccctccaacc accccgaaga
     1021 cagagctgca gtcgggcaag gcagacccga agcgggacgg gcgctccatg ggggagggcg
     1081 ggaagcctca catcgacttc ggcaacgtgg acattggtga gatcagccac gaggtaatgt
     1141 ccaacatgga gacctttgat gtggctgagt tggaccagta cctgccgccc aatgggcacc
     1201 caggccacgt gagcagctac tcagcagccg gctatgggct gggcagtgcc ctggccgtgg
     1261 ccagtggaca ctccgcctgg atctccaagc caccaggcgt ggctctgccc acggtctcac
     1321 cacctggtgt ggatgccaaa gcccaggtga agacagagac cgcggggccc caggggcccc
     1381 cacactacac cgaccagcca tccacctcac agatcgccta cacctccctc agcctgcccc
     1441 actatggctc agccttcccc tccatctccc gcccccagtt tgactactct gaccatcagc
     1501 cctcaggacc ctattatggc cactcgggcc aggcctctgg cctctactcg gccttctcct
     1561 atatggggcc ctcgcagcgg cccctctaca cggccatctc tgaccccagc ccctcagggc
     1621 cccagtccca cagccccaca cactgggagc agccagtata tacgacactg tcccggcccg
     1681 atatcggtgg tggctcgggg ggaggagcga caaactttag cttgctgaag caagctggtg
     1741 acgttgagga gaatcccgga ccaggattca ccggtatggt gagcaagggc gaggagctgt
     1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1861 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1981 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2041 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2221 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2281 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2461 ggatcactct cggcatggac gagctgtaca agtaaacgcg tgggggaggc taactgaaac
     2521 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     2581 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     2641 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     2701 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     2761 ccctgccata gctcgagtgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
     2821 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
     2881 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
     2941 aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttaaacggg
     3001 ccctctagag tcggtgggcc tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg
     3061 cttcggtcgg agccatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc
     3121 gggcgtcagg caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg
     3181 acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag
     3241 gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg gagcacgacg
     3301 gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc caggaaccac
     3361 gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg cgcggccagc
     3421 cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg
     3481 acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg
     3541 ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac ccgctcgatg
     3601 tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg
     3661 gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg
     3721 gtcatttcga gcctagggcc gggattctcc tccacgtcac cgcatgttag aagacttcct
     3781 ctgccctctc cgctgccaga tctgaggccc tgtgggagga agagaagagg tcaggccgaa
     3841 gttcctatac tatttgaaga ataggaactt caagcttgcc ccactgtggg gtggagggga
     3901 cagataaaag tacccagaac cagagccaca ttaaccggcc ctgggaatat aaggtggtcc
     3961 cagctcgggg acacaggatc cctggaggca gcaaacatgc tgtcctgaag tggacatagg
     4021 ggcccgggtt ggaggaagaa gactagctga gctctcggac ccctggaaga tgccatgaca
     4081 gggggctgga agagctagca cagactagag aggtaagggg ggtaggggag ctgcccaaat
     4141 gaaaggagtg agaggtgacc cgaatccaca ggagaacggg gtgtccaggc aaagaaagca
     4201 agaggatgga gaggtggcta aagccaggga gacggggtac tttggggttg tccagaaaaa
     4261 cggtgatgat gcaggcctac aagaagggga ggcgggacgc aagggagaca tccgtcggag
     4321 aaggccatcc taagaaacga gagatggcac aggccccaga aggagaagga aaagggaacc
     4381 cagcgagtga agacggcatg gggttgggtg agggaggaga gatgcccgga gaggacccag
     4441 acacggggag gatccgctca gaggacatca cgtggtgcag cgccgagaag gaagtgctcc
     4501 ggaaagagca tccttgggca gcaacacagc agagagcaag gggaagaggg agtggaggaa
     4561 gacggaacct gaaggaggcg gcagggaagg atctgggcca gccgtagagg tgacccaggc
     4621 cacaagctgc agacagaaag cggcacaggc ccaggggaga gaatgctggt cagagaaagc
     4681 aggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     4741 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4801 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4861 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4921 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4981 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5041 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5101 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5161 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5221 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5281 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     5341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7141 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7201 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7261 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     7321 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7381 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7441 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7501 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggaga
     7561 gcagagccag gaacccctgt agggaagggg caggagagcc aggggcatga gatggtggac
     7621 gaggaagggg gacagggaag cctgagcgcc tctcctgggc ttgccaagga ctcaaaccca
     7681 gaagcccaga gcagggcctt agggaagcgg gaccctgctc tgggcggagg aatatgtccc
     7741 agatagcact ggggactctt taaggaaaga aggatggaga aagagaaagg gagtagaggc
     7801 ggccacgacc tggtgaacac ctaggacgca ccattctcac aaagggagtt ttccacacgg
     7861 acacccccct cctcaccaca gccctgccag gacggggctg gctactggcc ttatctcaca
     7921 ggtaaaactg acgcacggag gaacaatata aattggggac tagaaaggtg aagagccaaa
     7981 gttagaactc aggaccaact tattctgatt ttgtttttcc aaactgcttc tcctcttggg
     8041 aagtgtaagg aagctgcagc accaggatca gtgaaacgca ccagacagcc gcgtcagagc
     8101 agctcaggtt ctgggagagg gtagcgcagg gtggccactg agaaccgggc aggtcacgca
     8161 tccccccctt ccctcccacc ccctgccaag ctctccctcc caggatcctc tctggctcca
     8221 tcgtaagcaa accttagagg ttctggcaag gagagagatg gctccaggaa atgggggtgt
     8281 gtcaccagat aaggaatctg cctaacagga ggtgggggtt agacccaata tcaggagact
     8341 aggaaggagg aggcctaagg atggggcttt tctgtcacca atcctgtccc tagtagcggc
     8401 cgcgaagttc ctatactttc tagagaatag gaacttcttc gaaccataga gcccaccgca
     8461 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     8521 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     8581 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     8641 acagatggct ggcaactaga aggcacagtc gaggcactag tttacccggg gagcatgtca
     8701 aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc gtcaagggca
     8761 tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt cggccggccc gccgctttcg
     8821 cactttagct gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt
     8881 tcggctccct gccggtcgaa cagctcaatt gcttgtctca gaagtggggg catagaatcg
     8941 gtggtaggtg tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag
     9001 cccagtgtaa agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct
     9061 tgctgacaca ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg
     9121 gtgccgagat gcactttagc cccgtcgcga tgtgagagga gagcacagcg gaatgacttg
     9181 gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca gggggcagaa gtgggtatga
     9241 tgcctgtcca gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc
     9301 cagtacaggg taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct
     9361 tcgataccga ctccattgag taattccaga gcgccgttta tgactttgct cttgtccagt
     9421 ctagacatga attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg
     9481 agctgtagga aaaagaagaa ggcatgaaca tggttagcag aggctctaga gccgccggtc
     9541 acacgccaga agccgaaccc cgccctgccc cgtccccccc gaaggcagcc gtccccccgc
     9601 ggacagcccc gaggctggag agggagaagg ggacggcggc gcggcgacgc acgaaggccc
     9661 tccccgccca tttccttcct gccggcgccg caccgcttcg ccccgcgccc gctagagggg
     9721 gtgcggcggc gcctcccaga tttcggctcc gcacagattt gggacaaagg aagtccctgc
     9781 gccctctcgc acgattacca taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg
     9841 ccggcgctcc gggggccgcc gcgcccctcc cccgagccct ccccggcccg aggcggcccc
     9901 gccccgcccg gcacccccac ctgccgccac cccccgcccg gcacggcgag ccccgcgcca
     9961 cgccccgtac ggagccccgc acccgaagcc gggccgtgct cagcaactcg gggagggggg
    10021 tgcagggggg gttgcagccc gaccgacgcg cccacacccc ctgctcaccc ccccacgcac
    10081 acaccccgca cgcagccttt gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc
    10141 ccggccgcgc tcccctcgcg cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag
    10201 cgctcacagc cgccgggcag cgcggagccg cacgcggcgc tccccacgca cacacacacg
    10261 cacgcacccc ccgagccgct ccccccgcac aaagggccct cccggagccc ctcaaggctt
    10321 tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga
    10381 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc
    10441 gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc
    10501 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
    10561 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
    10621 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccc cgcccccatc
    10681 gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag
    10741 atggggagag tgaagcagaa cgtggggctc acctcgacca tcatggtaat agcgatgact
    10801 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
    10861 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
    10921 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
    10981 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
    11041 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
    11101 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagctag tcaataatca
    11161 atgtcgacga tgatgctgta cactcgaaac ttttttcgaa ggtccgaggt tctagacgag
    11221 tttac
//
