LOCUS       sequence-229031-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..160
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaatacg gacctttaat
       61 tcaacccaac acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta
      121 attaaaatac tatactgtaa attacatttt atttacaatc actcgacgga tccttaagaa
      181 ggagatatac tatggcgacc ctggaaaagc tgatgaaggc cttcgagtcc ctcaagtcct
      241 tccaacagca gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac
      301 agcagcaaca gcaacaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc
      361 aacagcaaca acagcagcaa cagcaacaac agcagcaaca gcaacaacag cagcaacagc
      421 aacaacagca gcaacagcaa caacagcagc aacagcaaca acaacagcag caacagcaac
      481 aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag caacaacagc
      541 agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac
      601 agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac
      661 aacagcagca acagcaaccg ccaccgccgc cgccgccgcc gccgcctcct cagcttcctc
      721 agccgccgcc gcaggcacag ccgctgctgc ctcagccgca gccgcccc
//
