LOCUS       sequence-229032-        9788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of Sox10 (generated 
            from plasmid #20321).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9788)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 9788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: FUW-TetON-Sox10
FEATURES             Location/Qualifiers
     source          1..9788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        74..453
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        455..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             671..851
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    898..1023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1516..1749
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1934..1978
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2276..2393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2450..2468,2469..2491,2492..2510,2511..2533,2534..2552,
                     2553..2575,2576..2594,2595..2617,2618..2636,2637..2659,
                     2660..2678,2679..2701,2702..2720)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2753..2791
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2788..2812
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4316..4904
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4369..4389)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4787..4798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4907..4923)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4908..4924)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5429..5609
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5635..5652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5641..5865
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5911..6339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5998..6017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6208..6229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6348..6368)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6353..6682
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6533..6668
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6595..6614
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6730..6777
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6796..7170
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7300..7421
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7337..7356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7391..7410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7470..7486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7470..7486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7483..7505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7494..7510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7518..7524,7525..7542,7543..7548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7563..7584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7701..7718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7872..8460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7952..7971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8631..9422,9423..9491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9254..9273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9492..9596)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9671..9690)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
       61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      661 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      721 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      781 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
      841 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
      901 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
      961 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1021 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1081 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1141 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1201 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1261 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1321 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1381 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1441 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1501 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1561 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1621 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1681 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1741 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1801 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     1861 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     1921 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     1981 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2041 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2101 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2161 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2221 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2281 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2341 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2401 attacaggga cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt
     2461 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2521 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2581 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2641 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2701 ctccctatca gtgatagaga aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg
     2761 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     2821 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg
     2881 ccccgaattc atggcggagg agcaggacct atcggaggtg gagctgagcc ccgtgggctc
     2941 ggaggagccc cgctgcctgt ccccggggag cgcgccctcg ctagggcccg acggcggcgg
     3001 cggcggatcg ggcctgcgag ccagcccggg gccaggcgag ctgggcaagg tcaagaagga
     3061 gcagcaggac ggcgaggcgg acgatgacaa gttccccgtg tgcatccgcg aggccgtcag
     3121 ccaggtgctc agcggctacg actggacgct ggtgcccatg cccgtgcgcg tcaacggcgc
     3181 cagcaaaagc aagccgcacg tcaagcggcc catgaacgcc ttcatggtgt gggctcaggc
     3241 agcgcgcagg aagctcgcgg accagtaccc gcacctgcac aacgctgagc tcagcaagac
     3301 gctgggcaag ctctggaggc tgctgaacga aagtgacaag cgccccttca tcgaggaggc
     3361 tgagcggctc cgtatgcagc acaagaaaga ccacccggac tacaagtacc agcccaggcg
     3421 gcggaagaac gggaaggccg cccagggcga ggcggagtgc cccggtgggg aggccgagca
     3481 aggtgggacc gccgccatcc aggcccacta caagagcgcc cacttggacc accggcaccc
     3541 aggagagggc tcccccatgt cagatgggaa ccccgagcac ccctcaggcc agagccatgg
     3601 cccacccacc cctccaacca ccccgaagac agagctgcag tcgggcaagg cagacccgaa
     3661 gcgggacggg cgctccatgg gggagggcgg gaagcctcac atcgacttcg gcaacgtgga
     3721 cattggtgag atcagccacg aggtaatgtc caacatggag acctttgatg tggctgagtt
     3781 ggaccagtac ctgccgccca atgggcaccc aggccacgtg agcagctact cagcagccgg
     3841 ctatgggctg ggcagtgccc tggccgtggc cagtggacac tccgcctgga tctccaagcc
     3901 accaggcgtg gctctgccca cggtctcacc acctggtgtg gatgccaaag cccaggtgaa
     3961 gacagagacc gcggggcccc aggggccccc acactacacc gaccagccat ccacctcaca
     4021 gatcgcctac acctccctca gcctgcccca ctatggctca gccttcccct ccatctcccg
     4081 cccccagttt gactactctg accatcagcc ctcaggaccc tattatggcc actcgggcca
     4141 ggcctctggc ctctactcgg ccttctccta tatggggccc tcgcagcggc ccctctacac
     4201 ggccatctct gaccccagcc cctcagggcc ccagtcccac agccccacac actgggagca
     4261 gccagtatat acgacactgt cccggcccta agaattcgat atcaagctta tcgataatca
     4321 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     4381 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     4441 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     4501 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4561 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4621 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4681 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     4741 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4801 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4861 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     4921 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     4981 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5041 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5101 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     5161 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     5221 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     5281 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     5341 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     5401 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     5461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5581 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     5641 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5701 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5761 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5821 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5881 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5941 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6001 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6061 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6121 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6181 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6241 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6301 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6361 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6421 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6481 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6601 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6661 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6721 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     6781 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     6841 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     6901 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     6961 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7021 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7081 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     7141 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     7201 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7261 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7441 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7501 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7561 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7621 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7681 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7741 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7801 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8281 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8341 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8401 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8461 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8521 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9481 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9541 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9601 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     9661 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     9721 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     9781 tgaccgac
//
