LOCUS       sequence-229033-        9107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid for tet-inducible expression of GFP (generated 
            from plasmid #20321).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Garcia-Leon JA, Kumar M, Boon R, Chau D, One J, Wolfs E, Eggermont 
            K, Berckmans P, Gunhanlar N, de Vrij F, Lendemeijer B, Pavie B, 
            Corthout N, Kushner SA, Davila JC, Lambrichts I, Hu WS, Verfaillie 
            CM
  TITLE     SOX10 Single Transcription Factor-Based Fast and Efficient 
            Generation of Oligodendrocytes from Human Pluripotent Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Feb 13;10(2):655-672. doi: 
            10.1016/j.stemcr.2017.12.014. Epub 2018 Jan 11.
  PUBMED    29337119
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2017.12.014"; journalName: "Stem Cell Reports"; 
            date: "2018-02-13- 13"; volume: "10"; issue: "2"; pages: "655-672"
COMMENT     Sequence Label: FUW-TetON-GFP
FEATURES             Location/Qualifiers
     source          1..9107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        272..310
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     307..331
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(410..412,413..415,416..1129)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(410..412,413..415,416..1126)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(455..476)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(716..735)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1063..1084
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1154..1742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1207..1227)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1625..1636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1745..1761)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1746..1762)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2267..2447
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2473..2490)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2479..2703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2749..3177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2836..2855)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3046..3067
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3186..3206)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3191..3520
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3371..3506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3433..3452
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3568..3615
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3634..4008
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4138..4259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4175..4194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4229..4248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4308..4324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4308..4324)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4321..4343)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4332..4348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4356..4362,4363..4380,4381..4386))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4401..4422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4539..4556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4710..5298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4790..4809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5469..6260,6261..6329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6092..6111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6330..6434)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6509..6528)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6700..7079
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7081..7279
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7297..7477
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7524..7649
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8142..8375
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8560..8604
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8902..9019
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9076..9094,9095..9107,1..10,11..29,30..52,53..71,
                     72..94,95..113,114..136,137..155,156..178,179..197,198..220,
                     221..239)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
       61 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      121 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      181 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
      241 aagtgaaagt cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat
      301 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      361 cctccataga agacaccggg accgatccag cctccgcggc cccgaattca tggtgagcaa
      421 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      481 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      541 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      601 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      661 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      721 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      781 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      841 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      901 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      961 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     1021 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1081 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag aattcgatat
     1141 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     1201 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     1261 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     1321 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     1381 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     1441 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     1501 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     1561 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     1621 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     1681 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     1741 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     1801 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     1861 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     1921 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     1981 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     2041 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     2101 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     2161 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     2221 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     2281 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2341 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2401 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     2461 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2521 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2581 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2641 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     2701 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     2761 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2821 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2881 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2941 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3001 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3061 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3121 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     3181 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3241 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3421 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     3481 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     3541 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     3601 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     3661 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     3721 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     3781 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     3841 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     3901 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     3961 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     4021 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     4081 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     4141 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4201 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4261 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     4321 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4381 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4441 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4501 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4561 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4621 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5341 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5401 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5461 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5521 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5581 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5641 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5701 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5761 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5821 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5881 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5941 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6001 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6061 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     6121 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6181 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6241 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6301 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6361 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6421 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     6481 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     6541 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     6601 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     6661 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     6721 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     6781 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     6841 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     6901 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     6961 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     7021 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     7081 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     7141 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     7201 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     7261 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     7321 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7381 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7441 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7501 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7561 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7621 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7681 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7741 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7801 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7861 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7921 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7981 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     8041 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     8101 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     8161 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     8221 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     8281 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     8341 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     8401 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     8461 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     8521 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     8581 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     8641 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     8701 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     8761 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8821 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     8881 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     8941 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     9001 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaga
     9061 tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcg
//
