LOCUS       sequence-229055-        2715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2715)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U6-MosIR
FEATURES             Location/Qualifiers
     source          1..2715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(385..633)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(443..462)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(613..633)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(733..750)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(904..1492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(984..1003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1663..2454,2455..2523))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2286..2305
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2524..2628)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2696..2714
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag aaaaaaggta cctaggctgt tggagtcttc gggcgtgcgc gtgctggcag
       61 ccacaacccg aactatgttg tcgtggcgta gtcttgcaat gttcagttca gcccagaaac
      121 tccgctggga tgcacgtagg tccttggtgc aagggtgatt ccaaaagagt aaaactttgg
      181 taaaccagat tatttatcca tctaccccag tctgtgttta cccccctttc ttgtttactt
      241 gcttgatggt gcaccaagga cctacgtgca tcccagcgga gtttctgggc tgaactgaac
      301 attgcaagac tacgccacga caacatagtt cgggttgtgg ctgccagcac gcgcacgccc
      361 gaagactcca acagcctagg taccggtgtt tcgtcctttc cacaagatat ataaagccaa
      421 gaaatcgaaa tactttcaag ttacggtaag catatgatag tccattttaa aacataattt
      481 taaaactgca aactacccaa gaaattatta ctttctacgt cacgtatttt gtactaatat
      541 ctttgtgttt acagtcaaat taattccaat tatctctcta acagccttgt atcgtatatg
      601 caaatatgaa ggaatcatgg gaaataggcc ctcaattgct ttccagtcgg gaaacctgtc
      661 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      721 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      781 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      841 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      901 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      961 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1021 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1081 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1141 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1201 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1261 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1321 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     1381 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1441 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1501 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1561 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1621 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1681 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1741 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1801 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1861 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1921 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1981 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2041 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2101 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2161 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2221 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2281 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2341 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2401 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2461 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2521 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2581 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2641 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2701 gcgtatcacg aggcc
//
