LOCUS       sequence-229058-        8025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive integrase cassette - Bxb1-Tp901-Int5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 3511 to 3545)
  AUTHORS   Guiziou S, Mayonove P, Bonnet J
  TITLE     Hierarchical composition of reliable recombinase logic devices.
  JOURNAL   Nat Commun. 2019 Jan 28;10(1):456. doi: 10.1038/s41467-019-08391-y.
  PUBMED    30692530
REFERENCE   2  (bases 1 to 8025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-28"; volume: "10(1):456. doi"; pages: " 
            10.1038/s41467-019-08391-y"
COMMENT     Sequence Label: SG-C-Int-11
FEATURES             Location/Qualifiers
     source          1..8025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             117..128
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..1637
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="RALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDV
                     SGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVV
                     SATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYL
                     PTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGR
                     EPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAEL
                     VKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVA
                     MAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGS
                     PQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMN
                     VRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      1685..1756
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1772..1799
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3511..3545
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             5268..5279
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5721..5738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6028..6047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6643..6737)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             join(6835..6903,6904..7695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7699..7801)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(7978..8021)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gatactgtta cttaccgatt attgtgaaga accagaccgt ttgttgtagc acactcggcg
       61 aaaatctgaa tagtaacttc ttgacaatta atcatccggc tcgtaattta tgtggaaaag
      121 aggagaaata ctaggtgaga gccctggtag tcatccgcct gtcccgcgtc accgatgcta
      181 cgacttcacc ggagcgtcag ctggagtctt gccagcagct ctgcgcccag cgcggctggg
      241 acgtcgtcgg ggtagcggag gatctggacg tctccggggc ggtcgatccg ttcgaccgga
      301 agcgcagacc gaacctggcc cggtggcttg cgttcgagga gcaaccgttt gacgtgatcg
      361 tggcgtaccg ggtagatcgg ttgacccgat cgatccggca tcttcagcag ctggtccact
      421 gggccgagga ccacaagaag ctggtcgtct ccgcgaccga agcgcacttc gatacgacga
      481 cgccgtttgc ggcggtcgtc atcgcgctta tgggaacggt ggcgcagatg gaattagaag
      541 cgatcaaaga gcggaaccgt tcggctgcgc atttcaatat ccgcgccggg aaataccgag
      601 ggtccctgcc gccgtgggga tacctgccta cgcgcgtgga cggggagtgg cgactggtgc
      661 cggaccctgt gcagcgagag cgcatcctcg aggtgtatca ccgcgtcgtc gacaaccacg
      721 agccgctgca tctggtggcc cacgacctga accggcgtgg tgtcctgtcg ccgaaggact
      781 acttcgcgca gctgcaaggc cgcgagccgc agggccggga gtggtcggct accgcgctga
      841 agcgatcgat gatctccgag gcgatgctcg ggtacgcgac tctgaacggt aagaccgtcc
      901 gagacgacga cggagccccg ctggtgcggg ctgagccgat cctgacccgt gagcagctgg
      961 aggcgctgcg cgccgagctc gtgaagacct cccgggcgaa gcccgcggtg tctaccccgt
     1021 cgctgctgct gcgggtgttg ttctgcgcgg tgtgcgggga gcccgcgtac aagttcgccg
     1081 ggggaggacg taagcacccg cgctaccgct gccgctcgat ggggttcccg aagcactgcg
     1141 ggaacggcac ggtggcgatg gccgagtggg acgcgttctg cgaggagcag gtactggatc
     1201 tgctcgggga cgcggagcgt ctggagaaag tctgggtagc gggctcggac tccgcggtcg
     1261 aactcgcgga ggtgaacgcg gagctggtgg acctgacgtc gctgatcggc tccccggcct
     1321 accgggcggg ctctccgcag cgagaagcac tggatgcccg tattgcggcg ctggccgcgc
     1381 ggcaagagga gctggagggc ctggaggctc gcccgtctgg ctgggagtgg cgcgagaccg
     1441 ggcagcggtt cggggactgg tggcgggagc aggacaccgc ggcaaagaac acctggcttc
     1501 ggtcgatgaa cgttcggctg acgttcgacg tccgcggcgg gctgactcgc acgatcgact
     1561 tcggggatct tcaggagtac gagcagcatc tcaggctcgg cagcgtggtc gaacggctac
     1621 acaccgggat gtcgtaaata agttgtgccg tatccagcgg ttaccaataa tagtctccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttatatagta aagttccaat aagactccag gtatctgtcc gtgtaaaaaa gagtattgac
     1861 ttcgcatctt tttgtacctt aatgtgtgga tcacacagga aagtactaga tgactaagaa
     1921 agtagcaatc tatacacgag tatccactac taaccaagca gaggaaggct tctcaattga
     1981 tgagcaaatt gaccgtttaa caaaatatgc tgaagcaatg gggtggcaag tatctgatac
     2041 ttatactgat gctggttttt caggggccaa acttgaacgc ccagcaatgc aaagattaat
     2101 caacgatatc gagaataaag cttttgatac agttcttgta tataagctag accgcctttc
     2161 acgtagtgta agagatactc tttatcttgt taaggatgtg ttcacaaaaa ataaaataga
     2221 ctttatctcg cttaatgaaa gtattgatac ttcttctgct atgggtagct tgtttctcac
     2281 tattctttct gcaattaatg agtttgaaag agagaatata aaagaacgca tgactatggg
     2341 taaactaggg cgagcgaaat ctggtaagtc tatgatgtgg actaagacag cttttgggta
     2401 ttaccacaac agaaagacag gtatattaga aattgttcct ttacaagcta caatagttga
     2461 acaaatattc actgattatt tatcaggaat atcacttaca aaattaagag ataaactcaa
     2521 tgaatctgga cacatcggta aagatatacc gtggtcttat cgtaccctaa gacaaacact
     2581 tgataatcca gtttactgtg gttatatcaa atttaaggac agcctatttg aaggtatgca
     2641 caaaccaatt atcccttatg agacttattt aaaagttcaa aaagagctag aagaaagaca
     2701 acagcagact tatgaaagaa ataacaaccc tagacctttc caagctaaat atatgctgtc
     2761 agggatggca aggtgcggtt actgtggagc acctttaaaa attgttcttg gccacaaaag
     2821 aaaagatgga agccgcacta tgaaatatca ctgtgcaaat agatttcctc gaaaaacaaa
     2881 aggaattaca gtatataatg acaataaaaa gtgtgattca ggaacttatg atttaagtaa
     2941 tttagaaaat actgttattg acaacctgat tggatttcaa gaaaataatg actccttatt
     3001 gaaaattatc aatggcaaca accaacctat tcttgatact tcgtcattta aaaagcaaat
     3061 ttcacagatc gataaaaaaa tacaaaagaa ctctgatttg tacctaaatg attttatcac
     3121 tatggatgag ttgaaagatc gtactgattc ccttcaggct gagaaaaagc tgcttaaagc
     3181 taagattagc gaaaataaat ttaatgactc tactgatgtt tttgagttag ttaaaactca
     3241 gttgggctca attccgatta atgaactatc atatgataat aaaaagaaaa tcgtcaacaa
     3301 ccttgtatca aaggttgatg ttactgctga taatgtagat atcatattta aattccaact
     3361 cgctaccggt taaattgaac ctctactacg agtgagttga gattacagcc ttactcggta
     3421 ccaaattcca gaaaagagac gctttcgagc gtcttttttc gttttggtcc cttacgctat
     3481 tattgaagcc agtctgttac cgaagtgaaa ttgacggcta gctcagtcct aggtacagtg
     3541 ctagccagag gaaggaggct cgatgcctgg tatgaccacc gaaaccggtc cggatccggc
     3601 aggtctgatt gacctgtttt gtcgtaaaag caaagcagtt aaaagccgtg caaatggtgc
     3661 aggtcagcgt cgtaaacaag aaattagcat tgcagcacaa gaaaccctgg gtcgtaaagt
     3721 tgcagcactg ctgggtatgc aggttcgtca tgtttggaaa gaagttggta gcgcaagccg
     3781 ttttcgtaaa ggtaaagcac gtgatgatca gagcaaagca ctgaaagccc tggaaagcgg
     3841 tgaagttggt gcactgtggt gttatcgtct ggatcgttgg gatcgtggtg gtgccggtgc
     3901 aattctgaaa attatcgaac cggaagatgg tatgcctcgt cgtctgctgt ttggttggga
     3961 tgaagatacc ggtagtccgg ttctggatag caccaataaa cgtgatcgcg gtgaactgat
     4021 tcgtcgtgca gaagaagcac gcgaagaagc agaaaaactg agcgaacgtg ttcgtgatac
     4081 caaagcacat cagcgtgaaa atggtgaatg ggttaatgcc cgtgcaccgt atggtctgcg
     4141 tgttgttctg gttaccgtta gtgatgaaga gggtgatgaa tatgatgaac gtaaactggc
     4201 agcagatgat gaagatgcgg gtggtcctga tggtctgacc aaagcagaag cagcccgtct
     4261 ggtttttacc ctgccggtta ccgatcgtct gagctatgca ggcaccgcac atgcaatgaa
     4321 tacccgtgaa attccgagcc cgaccggtgg tccgtggatt gcagttaccg tgcgtgatat
     4381 gattcagaat ccggcatatg cgggttggca gaccacaggt cgtcaggatg gtaaacagcg
     4441 tcgtctgacc ttttataacg gtgaaggtaa acgtgttagc gttatgcatg gtcctccgct
     4501 ggtgaccgat gaagaacaag aagccgcaaa agcagccgtt aaaggtgaag atggtgttgg
     4561 tgttccgctg gatggtagcg atcatgatac ccgtcgcaaa catctgctga gcggtcgtat
     4621 gcgttgtccg ggttgtggtg gtagctgtag ctatagcggt aatggttatc gttgttggcg
     4681 tagcagtgtg aaaggtggtt gtccggcacc gacctatgtt gcacgtaaaa gcgttgaaga
     4741 atatgttgca tttcgttggg cagcaaaatt agcagcaagc gaaccggatg atccgtttgt
     4801 tattgcagtt gcagatcgct gggcagcact gacccatccg caggcaagcg aagatgaaaa
     4861 gtatgcaaaa gccgcagttc gtgaagccga aaaaaatctg ggtcgcctgc tgcgtgatcg
     4921 tcagaatggt gtttatgatg gtccggcaga acagtttttt gcccctgcat atcaagaagc
     4981 actgagcacc ttgcaggcag ccaaagatgc agttagcgaa agcagcgcaa gcgcagcagt
     5041 tgatgttagc tggattgttg atagcagcga ttatgaagaa ctgtggctgc gtgcaacccc
     5101 gaccatgcgt aatgcaatta ttgatacctg catcgatgaa atttgggttg caaaaggcca
     5161 gcgtggtcgt ccgtttgatg gtgatgaacg cgttaaaatc aaatgggcag cccgtaccta
     5221 aaacgattac ggattgctct actgtgactg aagtttacaa cccaattatt gaaggccgct
     5281 aacgcggcct ttttttgttt ctggtctccc tgaggcacag agatttactt tattcacgac
     5341 ttcagatacg cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg
     5401 ttgctggata actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa
     5461 cggtttccct ctacaaataa ttttgtttaa ctttagtaat ttcaacaaaa taactaggat
     5521 tcgaaatacg gtctactaca gaagggtggt ttatcttact caacgacgaa caataaggcc
     5581 tccctaacgg ggggcctttt ttattgataa caaaaagatt tccctcgtca cgcagtaagt
     5641 atttatcgta gaagatcact tttatcggtt tccagaacag gtaagagcca atagtgatta
     5701 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5761 cgagcggtat cagctcactc aaaggcggta atcctagcta gggcgttcgg ctgcggcgag
     5821 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6301 ccactggtaa caggaagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6361 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6421 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6481 caaacaaacc accgctggta gcggtggttt tttgtttgca agcagcagat tacgcgcaga
     6541 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6601 gaaaactcac gttaagggat tttggtcatg acttgtgctt ggattctcac caataaaaaa
     6661 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     6721 atctatcagc aggagtccaa gcgagctcga tatcactaaa tacattcaaa tatctatccg
     6781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gaatatgagt
     6841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6901 gctcacccag aaacgctggt gaaagtaaaa gatgccgaag atcagttggg tgcacgtgtg
     6961 ggttacatcg aactggacct caacagcggt aagattcttg agagttttcg ccccgaagaa
     7021 cgtttcccaa tgatgagcac ttttaaagtt ctgctctgtg gcgcggtatt atcccgtatt
     7081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7141 tactcaccag tcacagaaaa gcatcttacg gacggcatga cagtacgcga attatgcagc
     7201 gctgccataa ccatgagtga taacacggcg gccaacttac ttctgacaac gatcggagga
     7261 ccgaaggagc ttaccgcttt tttgcacaac atgggtgatc atgtaactcg ccttgatcgt
     7321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7381 gctatggcaa caacgttgcg caaactctta actggcgaac ttcttactct cgcttcccgg
     7441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctccctg
     7681 attaagcatt ggtaataatt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     7741 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     7801 aacgtctcat tttcgccaga tatcgacgtc taagaaacca ttattatcat gacattaacc
     7861 tataaaaata ggcgtatcac gaggcccttt cgtcttcacc tcgagtgcca cctgacgtct
     7921 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt
     7981 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctgga
//
