LOCUS       sequence-229061-        2630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ssRNA expression for dsRNA production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2630)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U6-Mos-s
FEATURES             Location/Qualifiers
     source          1..2630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(300..548)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(358..377)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(528..548)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(648..665)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(819..1407)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(899..918)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1578..2369,2370..2438))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2201..2220
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2439..2543)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2611..2629
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag aaaaaagcgg ccgctaacaa catctaggga atacttgagg cacttcccca
       61 aactcagttg ttctctgcag ctgagaggct ccggtgagcg ggtggcaccg tagatgactt
      121 ggtgtagagt cacgttgccc ccaaactcca tgattatggt acctaggctg ttggagtctt
      181 cgggcgtgcg cgtgctggca gccacaaccc gaactatgtt gtcgtggcgt agtcttgcaa
      241 tgttcagttc agcccagaaa ctccgctggg atgcacgtag gtccttggtg cgcggccgcg
      301 gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg
      361 gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat
      421 tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt
      481 ccaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat
      541 aggccctcaa ttgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      601 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      661 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      721 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      781 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      841 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      901 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      961 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1021 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1081 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1141 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1201 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1261 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1321 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1381 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1681 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2581 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
//
