LOCUS       sequence-229072-       10820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10820)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF1-MH.Bl-mDcrSOM
FEATURES             Location/Qualifiers
     source          1..10820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..1191
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          240..1182
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1139..1159
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1223..1242
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1223..1241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7010..7039
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7055..7072
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7095..7112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7101..7325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7666..7685
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7805..8203
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8361..8482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8398..8417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8452..8471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8607..9195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8687..8706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9366..10157,10158..10226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9989..10008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10234..10338)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10365..10793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10452..10471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10662..10683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
       61 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaagtggcgc ggggtaaact
      121 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      181 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      241 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
      301 cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
      361 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
      421 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
      481 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
      541 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
      601 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
      661 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
      721 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
      781 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
      841 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
      901 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
      961 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1021 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1081 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     1141 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg acgcgtccca
     1201 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
     1261 taagcttacc atggcaggcc tgcagctcat gacccctgct tcctcaccaa tgggtccttt
     1321 ctttggactg ccatggcaac aagaagcaat tcatgataac atttatacgc caagaaaata
     1381 ccaggttgag ctgctggaag cagctctgga ccataacaca attgtgtgct tgaacactgg
     1441 ctcagggaag acgttcatcg cggtcctgct caccaaagag ctggcccatc agatcagggg
     1501 cgacctcaac ccgcatgcaa aaaggaccgt gttcctcgtc aactctgcaa accaggttgc
     1561 tcaacaagtg tcagccgtca gaactcactc ggatctcaag gttggagagt actcagacct
     1621 agaagtaaat gcatcttgga caaaagagag atggagccaa gagtttacta agcaccaggt
     1681 tctcattatg acttgctatg tcgccttgac tgttttgaaa aatggttact tatcactgtc
     1741 agacattaac cttttggtgt ttgatgagtg tcatcttgcg attctagacc acccctatcg
     1801 tgaaattatg aagctctgtg agagttgtcc atcatgccct cgaattttgg gactaactgc
     1861 ttccatttta aatgggaaat gtgatccaga ggaattggaa gagaagattc agaaactgga
     1921 gcgaattctc aggagtgacg ctgagacggc gactgacttg gtggtcctgg acagatacac
     1981 ttctcagcct tgtgagattg tggtagactg tggaccgttt actgacagaa gtgggctgta
     2041 tgagagattg ctgatggagt tagaagcagc acttgatttt atcaatgatt gtaatgtagc
     2101 tgtacattca aaggaaagag attctacttt gatttccaaa caaatcctct cagactgccg
     2161 tgcagtgttg gttgttctgg gaccctggtg tgcagataaa gtagctggga tgatggtaag
     2221 agaacttcag aagtacatca aacatgagca agaggagcta cacaggaagt tcctattgtt
     2281 tacagacacc ttgttaagga aaatacacgc cctgtgtgaa gagtacttct cacctgcctc
     2341 acttgacctg aagtatgtaa ctcccaaagt catgaaactg ctcgagatcc tgcgcaaata
     2401 caagccctat gagcgacagc agtttgaaag tgttgagtgg tacaataaca ggaatcagga
     2461 taattacgtg tcctggagtg attccgagga tgacgatgat gacgaagaaa ttgaagaaaa
     2521 agaaaagcca gagacaaatt ttccttctcc gtttaccaat attttatgtg ggattatttt
     2581 tgtggaaaga agatacacag cagttgtcct aaacagattg ataaaggaag ctggcaaaca
     2641 agatccagag ctggcttata tcagcagcaa ctttataact ggacacggca ttggaaagaa
     2701 ccagcctcgc agcaaacaga tggaggcgga gttcagaaag caggaggagg tacttaggaa
     2761 atttcgagct catgagacca acctgctcat tgcaacaagt gttgtagaag agggtgtgga
     2821 tattcccaag tgcaatttgg tggttcgttt tgatttgcct acagagtatc gatcatatgt
     2881 ccagtctaaa ggacgggcac gagcgccgat ctctaattac gtaatgttag cagatacaga
     2941 caagataaag agctttgaag aagacctcaa gacctacaaa gctattgaaa agatcttaag
     3001 gaacaagtgt tcgaagtcag cggatggtgc tgaggcggac gtccatgctg gtgtagatga
     3061 tgaggatgct ttcccgccat atgtgctgag gcctgatgat gggggtcctc gagtcacaat
     3121 caacactgcc attggacaca tcaacagata ttgtgctcga ttaccaagtg atccgtttac
     3181 gcatctagct cccaaatgta gaacccgaga attgcctgat ggtacatttt attcaactct
     3241 ttatcttccg attaactcac ctcttcgagc ctccattgtt ggtccaccga tggacagtgt
     3301 acggttggct gaaagagtgg tagctctcat ttgctgtgaa aaactgcaca aaattggtga
     3361 actggatgaa cacttgatgc cagttgggaa agagactgtt aaatatgaag aagagctgga
     3421 cttacatgac gaggaggaga ccagtgttcc cgggagacca gggtccacaa aacgacggca
     3481 gtgctacccc aaagcaattc cagagtgctt gagggagagt taccccaaac ccgaccagcc
     3541 ttgttacctg tatgtgatag gaatggtctt aactaccccc ctccctgatg aactcaactt
     3601 caggcggcgg aagctctacc cacctgagga caccacaaga tgctttggga tcctgacagc
     3661 caaacccata cctcagattc ctcactttcc tgtgtataca cgctctggag aggtcaccat
     3721 atccatcgag ctgaagaagt ctggtttcac attgtctcaa caaatgctcg agctgattac
     3781 aagattgcac cagtacatat tctcacatat tctccggctt gagaagcctg ccctggagtt
     3841 taaacctaca ggcgcggagt cagcctactg tgttctacct cttaatgttg ttaatgactc
     3901 cggcactttg gacattgact ttaaattcat ggaggatatt gagaagtcgg aagctcggat
     3961 aggcattcct agcaccaagt attcaaagga aacaccgttt gtctttaagt tagaagatta
     4021 ccaagatgca gtgatcattc caagataccg caattttgat cagcctcatc gattttatgt
     4081 agctgatgta tatacggatc ttaccccact cagtaaattt ccttcccctg aatatgaaac
     4141 ttttgcagaa tattataaaa caaagtataa ccttgacctg accaatctca accagccact
     4201 gctggatgtt gaccacacat cttccagact gaaccttttg acacctcggc atttgaatca
     4261 gaaggggaaa gcacttcctc taagcagtgc tgagaagagg aaggccaaat gggaaagtct
     4321 gcagaacaaa cagatcctgg ttccggaact ctgtgctata catccaatcc cagcatcact
     4381 gtggagaaaa gcggtttgtc tccccagcat actgtatcgc cttcactgcc ttctgactgc
     4441 agaggaacta agagctcaga cagccagcga tgctggtgtg ggtgtcagat cacttcccgt
     4501 ggattttaga taccctaact tagacttcgg gtggaaaaaa tctattgata gcaagtcttt
     4561 catctcaagc tgtaactcct cgttggctga gagtgataat tactgtaagc acagcacaac
     4621 tgtagtccct gaacatgctg cacatcaagg tgctactaga ccctccttag aaaaccatga
     4681 ccaaatgtct gtgaactgca aaaggttgcc cgctgagtca cctgctaagc tccaaagtga
     4741 ggtttcaacg gatcttacag caattaatgg tctttcttac aataaaaacc ttgccaatgg
     4801 cagttatgat ttagttaaca gagacttttg ccaaggaaat cagctgaatt acttcaagca
     4861 ggaaatacct gtacaaccaa ctacctcata tcccattcag aatttgtaca attatgagaa
     4921 ccagcccaag cccagcaatg aatgtcctct actgagtaat acgtaccttg atgggaacgc
     4981 taacacatct acctcagatg gaagccccgc ggtgtccacg atgcctgcca tgatgaatgc
     5041 tgttaaagca ctcaaggaca ggatggactc tgagcagagc ccttctgttg ggtactcctc
     5101 aaggactctt ggccccaatc cgggactgat tctccaggct ttgacactgt caaatgccag
     5161 tgatgggttt aacctggagc ggcttgagat gcttggcgac tcctttttaa agcatgctat
     5221 caccacatat ctgttttgca cgtaccctga tgctcatgaa ggccgccttt catatatgag
     5281 aagcaaaaag gtcagcaact gtaacctgta tcgccttggc aagaagaagg gactgcccag
     5341 ccgcatggtg gtgtcgatat tcgatcctcc tgtgaattgg cttcctcctg gttatgtggt
     5401 aaaccaagac aaaagtaact cagagaagtg ggaaaaggat gaaatgacaa aagactgctt
     5461 gctggctaat ggcaaactgg gtgaggcctg tgaggaggag gaggacctga cgtggagggc
     5521 ccccaaggag gaggccgagg atgaggacga cttcctggag tatgaccagg agcatatcca
     5581 gtttatagac agcatgctca tgggctcggg cgccttcgtg aggaaaatct ctctgtcgcc
     5641 cttctcagcc tccgactctg catacgaatg gaagatgccc aagaaggcct ccttgggcag
     5701 tatgccgttt gcctcgggcc ttgaggattt tgattacagc tcttgggatg cgatgtgcta
     5761 tctggatcct agcaaagccg ttgaagagga tgactttgtg gtaggtttct ggaatccatc
     5821 agaagagaac tgtggcgtcg acacagggaa gcagtccatt tcttacgact tgcacacgga
     5881 gcagtgcatt gctgacaaga gcatagcgga ctgtgtggag gccctgctgg gctgctactt
     5941 aaccagctgc ggcgagaggg ctgctcagct cttcctctgt tcgctggggc tgaaggtgct
     6001 cccagtaatc aaaaggacca gccgggagaa ggccttggac cctgctcagg agaacggaag
     6061 cagccaacaa aagagccttt cagggagctg tgcctctcca gttggcccac gctcctctgc
     6121 agggaaagac ttggagtacg gctgcctgaa gatcccaccg aggtgcatgt ttgaccatcc
     6181 agacgcagag aaaaccctca accacctgat atctgggttt gaaacttttg agaagaaaat
     6241 taactataga ttcaagaata aggcttatct tctgcaggct tttacacacg cctcctacca
     6301 ctacaacact atcactgatt gttaccagcg cttagaattc ctgggagatg cgattttgga
     6361 ctacctcata accaagcacc tttatgaaga cccacggcag cattctccag gggtcttgac
     6421 tgacttgcgc tctgcccttg tcaataacac catctttgca tcattggctg tgaagtatga
     6481 ctaccacaag tactttaaag ctgtctctcc tgagctcttt catgttattg atgactttgt
     6541 gaagtttcag cttgagaaga acgaaatgca aggaatggac tctgagctta ggagatccga
     6601 ggaggatgag gagaaagaag aggatatcga agtccccaag gccatggggg acatttttga
     6661 gtctcttgct ggtgccattt atatggacag tgggatgtct ctggaggtgg tctggcaggt
     6721 gtactatccg atgatgcagc ctctaataga aaagttttct gcaaatgtgc ctcgttctcc
     6781 agtgagagaa ttgctcgaga tggaaccaga aactgccaag tttagcccag cggagagaac
     6841 ttatgatgga aaggtcagag tcaccgtgga agtggtggga aaggggaaat ttaaaggtgt
     6901 tggccggagc tacaggatcg ccaagtctgc tgcagcacga agggccctcc gcagcctcaa
     6961 agctaaccag cctcaggttc ctaacagctc gctagagggc ccgcggttcg aacaaaaact
     7021 catctcagaa gaggatctga atatgcatac cggtcatcat caccatcacc attgagttta
     7081 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     7141 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     7201 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     7261 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     7321 tatggcttct gaggcggaaa gaacccggta ggcgccaacc ggctccgttc tttggtggcc
     7381 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     7441 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     7501 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     7561 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     7621 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     7681 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     7741 gcccaagcta gcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     7801 aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     7861 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     7921 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     7981 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     8041 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     8101 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     8161 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg
     8221 agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8281 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8341 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8461 ccaaactcat caatgtatct tatcatgtct gtatgcatgg cggtaatacg gttatccaca
     8521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct
     9361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10141 accagcgttt cagggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10201 gcgacacgga aatgttgaat actcatcctc aggactctcc ctttttcaat attattgaag
    10261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
    10381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    10441 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    10501 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    10561 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    10621 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    10681 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    10741 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    10801 aatattaacg cttacaattt
//
