LOCUS       sequence-229074-        4069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dxs in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::dxs
FEATURES             Location/Qualifiers
     source          1..4069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     321..340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      578..672
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(2580..2666)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2679..2697)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2765..2869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2870..2938,2939..3730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3088..3107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3901..4069,1..420)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
       61 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      421 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      481 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      541 gataccgctc gccgcagccg aacgaccgag cgccttggac tcctgttgat agatccagta
      601 atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt
      661 attggtgaga atccagtgtg ctcttcgatg tcttttgata ttgcgaaata tccaaccctg
      721 gccctagttg actcgactca ggaattacgc ctgctgccga aagagagcct tccaaagctg
      781 tgcgatgagt tacgccgcta cctcctggat tctgttagtc gtagctccgg ccacttcgca
      841 tcgggactag gtaccgtcga actgacggtt gcactgcatt atgtgtataa caccccgttc
      901 gatcaattaa tttgggacgt cggtcatcaa gcatatccgc ataaaattct gaccggtcgt
      961 cgcgacaaga tcggcacaat tcgtcaaaaa ggtggattgc atcctttccc gtggcgcggc
     1021 gagtccgaat acgatgtgtt gagcgttggc cattcgtcaa cttctatcag cgctggtatt
     1081 ggtattgcgg tcgccgcgga gaaagaaggc aaaaatcggc gtacggtttg cgtgatcggg
     1141 gatggtgcaa tcaccgcagg catggcattc gaagcgatga accatgcggg ggacattcgt
     1201 ccggatatgc tagtgattct gaacgataac gaaatgagta tttccgagaa cgtgggggct
     1261 cttaataacc acttagcgca gctgctgagc ggtaaacttt attctagcct gcgcgaaggc
     1321 ggtaagaaag tgttctcagg cgtccctccg attaaagagc tccttaagcg tactgaggaa
     1381 cacattaagg gtatggtggt tccaggcacc ctgttcgaag aactgggttt taattacatt
     1441 ggtcctgtgg acggccatga cgtgttaggc ttaattacca cgttaaaaaa catgcgcgat
     1501 ctgaaaggac ctcagtttct ccacatcatg accaagaaag gtcgcggcta tgaaccggcc
     1561 gagaaagatc cgattacctt ccacgcagtt ccgaaattcg acccatcctc cggctgtctg
     1621 ccgaaaagca gcggaggcct gccgagctat tccaaaatct ttggtgactg gctgtgcgaa
     1681 actgcggcaa aagataacaa actgatggcc atcaccccgg caatgcgcga aggttcggga
     1741 atggtggaat tctctcgcaa gttcccagac cgttattttg acgttgctat cgccgagcaa
     1801 catgcagtaa cctttgcggc tggtctggca attggtggtt ataagccaat tgtggccatc
     1861 tattcaacgt tcttacaacg tgcatatgac caggtgctac atgatgtggc cattcagaaa
     1921 ctgccggtct tatttgccat cgatcgtgcg gggatcgttg gggcagatgg tcagacgcat
     1981 cagggcgcct tcgacctaag ttatctgcgc tgtatcccag aaatggtgat tatgacccct
     2041 agcgatgaga atgaatgtcg tcagatgtta tacaccggct atcattacaa cgacggacca
     2101 tccgctgtgc gctacccgcg cggaaacgcg gtgggcgttg aactgacccc gttagaaaaa
     2161 ttaccgatcg gaaaagggat tgttaaacgt cgcggagaaa aactcgctat tctgaacttc
     2221 ggcacactta tgcccgaagc ggctaaagta gcggaaagcc taaacgcaac cttggtggat
     2281 atgcgcttcg taaagccact ggacgaagcc ctaatcttag agatggccgc gtcacatgag
     2341 gcgttagtta cggtagagga aaacgctatt atgggcggag cgggtagtgg tgtgaacgaa
     2401 gttctgatgg ctcaccgcaa accggtgccg gtactgaaca tcggcctccc ggattttttc
     2461 attccccaag gtactcaaga ggaaatgcgc gccgagctcg gcttagatgc tgcaggaatg
     2521 gaagccaaaa ttaaggcctg gcttgcataa tgaagagcgt aagacctcta gggcggcgga
     2581 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     2641 ttcgactgag cctttcgttt tatttgatgc ccgaaagggc ctcgtgatac gcctattttt
     2701 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2761 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2821 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2881 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2941 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3001 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3061 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3121 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3181 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3241 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3301 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3361 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3421 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3481 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3541 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtccc gcggtatcat
     3601 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3661 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3721 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3781 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3841 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3901 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3961 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4021 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagtta
//
