LOCUS       sequence-229080-        2244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RBS RBSc13 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::RBSc13
FEATURES             Location/Qualifiers
     source          1..2244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..135
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      148..234
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(317..411)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      complement(569..1157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(649..668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1328..2119,2120..2188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1951..1970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2189..2244,1..49))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
       61 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      121 ggcgtatcac gaggcccttt cgggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
      241 ccctagaggt cttacgctct tcacatctcg catacctcct ttttctaaaa aacgtagtgg
      301 cgaagagcca cactggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
      361 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaaggcgct
      421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1441 ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag
     1501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2221 atcagggtta ttgtctcatg agcg
//
