LOCUS       sequence-229082-       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express Septin 2 tagged with GFP on C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  TITLE     Cooper Lab plasmids
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBOB-Septin 2 -GFP
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             84..128
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    380..496
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        519..822
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        823..1026
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1023..1047
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2195..2204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2201..2203,2204..2206,2207..2920)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2201..2203,2204..2206,2207..2917)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2246..2267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2507..2526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2854..2875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3056..3644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3109..3129)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3527..3538)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4051..4231
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4257..4274)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4263..4487
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4533..4961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4620..4639)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4830..4851
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4970..4990)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4975..5304
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5155..5290
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5217..5236
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5352..5399
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5418..5792
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5922..6043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5959..5978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6013..6032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6092..6108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6092..6108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6105..6127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6116..6132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6140..6146,6147..6164,6165..6170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6185..6206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6323..6340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6494..7082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6574..6593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7253..8044,8045..8113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7876..7895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8114..8218)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8293..8312)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8484..8863
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8865..9063
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9081..9261
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9308..9433
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9926..10159
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
       61 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
      121 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
      181 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
      241 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      301 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
      361 gattagtgaa cggatcaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
      421 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      481 caaaattcaa aattttatcg ataagcttgg gagttccgcg ttacataact tacggtaaat
      541 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      601 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      661 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      721 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      781 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      841 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      901 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      961 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     1021 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     1081 catagaagac accgactcta gaatgtctaa gcaacagcca actcagttta taaatccaga
     1141 aacacctggc tatgttggat ttgcaaacct ccccaatcaa gttcaccgaa aatcagtgaa
     1201 aaaaggtttt gagttcacac tgatggtggt cggtgaatca ggtctaggaa aatcgactct
     1261 cataaacagc ctattcctaa ctgatctgta cccagaaaga gtcatacctg gagcagcaga
     1321 aaaaattgaa agaactgtcc agattgaggc ttcaactgtt gaaattgaag agcgaggggt
     1381 caagctacgc ctgacagtgg tagatacccc tggctatggt gacgctatca actgcagaga
     1441 ttgttttaag acaattatct cctatattga tgagcaattt gagaggtacc tgcatgacga
     1501 gagcggcttg aacaggcggc acatcattga taatagggtg cattgttgct tttactttat
     1561 ttcacctttt ggacatggac ttaagccctt agatgtggcg tttatgaagg caatacacaa
     1621 caaggtgaat attgtgcctg tcattgcaaa agctgacact ctcaccctga aggaacggga
     1681 gcggctgaag aaaaggattc tggatgaaat tgaagaacat aacatcaaaa tctatcactt
     1741 acctgatgca gaatcagatg aagatgaaga ttttaaagag cagactagac ttctcaaggc
     1801 tagcatccca ttctctgtgg ttggatccaa tcagttgatt gaagccaaag gaaagaaggt
     1861 cagaggccgc ctctacccct ggggtgttgt ggaagtggag aacccagagc acaatgactt
     1921 tctgaagctg agaaccatgc tcatcaccca catgcaggat ctccaggagg tgacccagga
     1981 ccttcattat gaaaacttcc gttctgagag actcaagaga ggcggcagga aagtggagaa
     2041 tgaggacatg aataaagacc agatcttgct ggaaaaagaa gctgagctcc gccgcatgca
     2101 agagatgatt gcaaggatgc aggcgcagat gcagatgcag atgcagggcg gggatggcga
     2161 tggcggggct ctcgggcacc acgtgggacc ggtcgccacc atggtgagca agggcgagga
     2221 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2281 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2341 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2401 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2461 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2521 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2581 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2641 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2701 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2761 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2821 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2881 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg tcgagtttaa
     2941 acctggaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     3001 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct cagacaatca
     3061 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3121 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3181 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3241 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3301 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3361 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3421 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     3481 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3541 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3601 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg
     3661 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     3721 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     3781 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     3841 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     3901 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     3961 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     4021 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     4081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4201 cccttttagt cagtgtggaa aatctctagc agggccgctt taaacccgct gatcagcctc
     4261 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4321 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4381 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4441 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4501 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4561 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4621 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4681 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4741 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4801 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4861 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4921 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     4981 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5041 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5101 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5161 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5221 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5281 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5341 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     5401 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     5461 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     5521 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     5581 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     5641 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     5701 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     5761 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     5821 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5881 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5941 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6001 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6061 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6121 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7141 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8221 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     8281 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     8341 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     8401 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     8461 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     8521 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     8581 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     8641 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     8701 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     8761 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     8821 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     8881 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     8941 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     9001 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     9061 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     9121 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     9181 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     9241 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     9301 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     9361 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     9421 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     9481 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     9541 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     9601 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     9661 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9721 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     9781 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9841 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9901 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9961 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    10021 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    10081 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    10141 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    10201 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
//
