LOCUS       sequence-229086-       11529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express VE-cadherin tagged with GFP on C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Kim J, Cooper JA
  TITLE     Septins regulate junctional integrity of endothelial monolayers.
  JOURNAL   Mol Biol Cell. 2018 Jul 15;29(13):1693-1703. doi: 
            10.1091/mbc.E18-02-0136. Epub 2018 May 17.
  PUBMED    29771630
REFERENCE   2  (bases 1 to 11529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E18-02-0136"; journalName: "Mol Biol Cell"; date: 
            "2018-07-15- 15"; volume: "29"; issue: "13"; pages: "1693-1703"
COMMENT     Sequence Label: pBOB-VE-cadherin-GFP
FEATURES             Location/Qualifiers
     source          1..11529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             190..370
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    417..542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1035..1268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1453..1497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1749..1865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1888..2191
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2192..2395
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2392..2416
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             complement(4515..4526)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      4833..4842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4839..4841,4842..4844,4845..5558)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4839..4841,4842..4844,4845..5555)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4884..4905)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5145..5164)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5492..5513
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5694..6282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5747..5767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6165..6176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6689..6869
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6895..6912)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6901..7125
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7171..7599
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7258..7277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7468..7489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7608..7628)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7613..7942
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7793..7928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7855..7874
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7990..8037
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8056..8430
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8560..8681
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8597..8616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8651..8670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8730..8746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8730..8746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8743..8765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8754..8770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8778..8784,8785..8802,8803..8808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8823..8844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8961..8978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9132..9720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9212..9231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9891..10682,10683..10751))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10514..10533
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10752..10856)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10931..10950)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11122..11501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(11503..11529,1..172)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
       61 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      121 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
      181 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      241 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      301 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      361 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
      421 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
      481 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
      541 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
      601 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
      661 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
      721 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
      781 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
      841 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
      901 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
      961 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1021 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1081 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1141 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1201 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1261 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1321 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     1381 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     1441 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     1501 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     1561 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     1621 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     1681 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     1741 ggatcaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1801 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa
     1861 attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1921 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1981 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2041 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2101 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2161 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2221 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2281 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2341 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     2401 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     2461 ccgactctag aatgcagagg ctcatgatgc tcctcgccac atcgggcgcc tgcctgggcc
     2521 tgctggcagt ggcagcagtg gcagcagcag gtgctaaccc tgcccaacgg gacacccaca
     2581 gcctgctgcc cacccaccgg cgccaaaaga gagattggat ttggaaccag atgcacattg
     2641 atgaagagaa aaacacctca cttccccatc atgtaggcaa gatcaagtca agcgtgagtc
     2701 gcaagaatgc caagtacctg ctcaaaggag aatatgtggg caaggtcttc cgggtcgatg
     2761 cagagacagg agacgtgttc gccattgaga ggctggaccg ggagaatatc tcagagtacc
     2821 acctcactgc tgtcattgtg gacaaggaca ctggtgaaaa cctggagact ccttccagct
     2881 tcaccatcaa agttcatgac gtgaacgaca actggcctgt gttcacgcat cggttgttca
     2941 atgcgtccgt gcctgagtcg tcggctgtgg ggacctcagt catctctgtg acagcagtgg
     3001 atgcagacga ccccactgtg ggagaccacg cctctgtcat gtaccaaatc ctgaagggga
     3061 aagagtattt tgccatcgat aattctggac gtattatcac aataacgaaa agcttggacc
     3121 gagagaagca ggccaggtat gagatcgtgg tggaagcgcg agatgcccag ggcctccggg
     3181 gggactcggg cacggccacc gtgctggtca ctctgcaaga catcaatgac aacttcccct
     3241 tcttcaccca gaccaagtac acatttgtcg tgcctgaaga cacccgtgtg ggcacctctg
     3301 tgggctctct gtttgttgag gacccagatg agccccagaa ccggatgacc aagtacagca
     3361 tcttgcgggg cgactaccag gacgctttca ccattgagac aaaccccgcc cacaacgagg
     3421 gcatcatcaa gcccatgaag cctctggatt atgaatacat ccagcaatac agcttcatcg
     3481 tcgaggccac agaccccacc atcgacctcc gatacatgag ccctcccgcg ggaaacagag
     3541 cccaggtcat tatcaacatc acagatgtgg acgagccccc cattttccag cagcctttct
     3601 accacttcca gctgaaggaa aaccagaaga agcctctgat tggcacagtg ctggccatgg
     3661 accctgatgc ggctaggcat agcattggat actccatccg caggaccagt gacaagggcc
     3721 agttcttccg agtcacaaaa aagggggaca tttacaatga gaaagaactg gacagagaag
     3781 tctacccctg gtataacctg actgtggagg ccaaagaact ggattccact ggaaccccca
     3841 caggaaaaga atccattgtg caagtccaca ttgaagtttt ggatgagaat gacaatgccc
     3901 cggagtttgc caagccctac cagcccaaag tgtgtgagaa cgctgtccat ggccagctgg
     3961 tcctgcagat ctccgcaaca gacaaggaca taacaccacg aaacgtgaag ttcaaattca
     4021 ctttgaatac tgagaacaac tttaccctca cggataatca cgataacacg gccaacatca
     4081 cagtcaagta tgggcagttt gaccgggagc ataccaaggt ccacttccta cccgtggtca
     4141 tctcagacaa tgggatgcca agtcgcacgg gcaccagcac gctgaccgtg gccgtgtgca
     4201 agtgcaacga gcagggcgag ttcaccttct gcgaggatat ggccgcccag gtgggcgtga
     4261 gcatccaggc agtggtagcc atcttactct gcatcctcac catcacagtg atcaccctgc
     4321 tcatcttcct gcggcggcgg ctccggaagc aggcccgcgc gcacggcaag agcgtgccgg
     4381 agatccacga gcagctggtc acctacgacg aggagggcgg cggcgagatg gacaccacca
     4441 gctacgatgt gtcggtgctc aactcggtgc gccgcggcgg ggccaagccc ccgcggcccg
     4501 cgctggacgc ccggccttcc ctctatgcgc aggtgcagaa gccaccgagg cacgcgcctg
     4561 gggcacacgg agggcccggg gagatggcag ccatgatcga ggtgaagaag gacgaggcgg
     4621 accacgacgg cgacggcccc ccctacgaca cgctgcacat ctacggctac gagggctccg
     4681 agtccatagc cgagtccctc agctccctgg gcaccgactc atccgactct gacgtggatt
     4741 acgacttcct taacgactgg ggacccaggt ttaagatgct ggctgagctg tacggctcgg
     4801 acccccggga ggagctgctg tatggaccgg tcgccaccat ggtgagcaag ggcgaggagc
     4861 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     4921 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     4981 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     5041 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5101 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     5161 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     5221 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     5281 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     5341 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     5401 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     5461 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     5521 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgtc gagtttaaac
     5581 ctggaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     5641 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca gacaatcaac
     5701 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5761 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5821 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5881 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5941 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6001 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6061 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     6121 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6181 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6241 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt
     6301 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6361 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     6421 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     6481 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     6541 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     6601 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     6661 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     6721 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6781 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6841 cttttagtca gtgtggaaaa tctctagcag ggccgcttta aacccgctga tcagcctcga
     6901 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6961 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7021 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7081 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7141 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     7201 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     7261 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     7321 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     7381 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7441 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7501 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7561 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     7621 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7681 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7741 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7801 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7861 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7921 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7981 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     8041 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     8101 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     8161 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     8221 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     8281 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     8341 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     8401 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     8461 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8521 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8581 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8641 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8701 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8761 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8821 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8881 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8941 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9001 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9061 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9121 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9181 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9241 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9301 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9361 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9421 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9481 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9541 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9601 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9661 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9721 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9781 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9841 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9901 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9961 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10021 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10081 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10141 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10201 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10261 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10321 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10381 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10441 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10501 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10561 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10621 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10681 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10741 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10801 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10861 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
    10921 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
    10981 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
    11041 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
    11101 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
    11161 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
    11221 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
    11281 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
    11341 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
    11401 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
    11461 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
    11521 acatcaatg
//
