LOCUS       sequence-22909-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  modified pENTR vector for N-terminal 6x MYC tag fusion with your 
            gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE3n
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             154..183
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             193..222
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             232..261
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             271..300
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             310..339
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             373..402
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(476..575)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cttncngggc ccaatatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcacc tgntttttta taatgccaac tttgtacaaa aaagcaggct ttaaaggaac
      121 caattcagtc gacggtatcg atttaaagct atggagcaaa agctcatttc tgaagaggac
      181 ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag
      241 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg
      301 aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga gagcttgggc
      361 gacctcacca tggagcaaaa gctcatttct gaagaggact tgaattccgg aagcccggga
      421 tccggtacag atttcggaca tgcggccgca taagtagctg actcgagata tctagaccca
      481 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcnacgaa
      541 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc tctggcccgt
      601 gtctcaaaat ctctgatgtt acattgcaca agataaaaat
//
