LOCUS       sequence-229090-        3709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lcyB in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::lcyB
FEATURES             Location/Qualifiers
     source          1..3709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..95
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(314..334)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     terminator      complement(1643..1729)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1742..1760)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1828..1932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1933..2001,2002..2793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2151..2170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2964..3552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3453..3472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
       61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtgctcttcg atggatacgc
      121 ttctgaaaac gcctaataac cttgagtttc tgaatcccca tcatggtttt gcagtgaaag
      181 cgagcacctt ccgttccgag aaacatcata atttcgggag tcgcaaattc tgtgaaacac
      241 ttggccgctc ggtgtgtgtg aagggctcat cgtcggcctt attggaactt gtgccggaga
      301 ctaaaaaaga aaacttggat tttgaactgc ccatgtatga cccttctaaa ggagtggtgg
      361 ttgatcttgc cgtagtagga gggggcccgg cgggcctggc cgtagcccag caggtgagcg
      421 aagctggtct gagcgtctgc tctattgacc cgaatccaaa actgatctgg ccaaacaact
      481 acggcgtatg ggtggatgag tttgaggcga tggatctgct ggattgctta gatgcgacct
      541 ggtcgggcgc tgcagtgtat attgatgaca atactgcaaa agaccttcac cgcccttacg
      601 gtcgtgttaa ccgtaaacag ttaaaatcca aaatgatgca gaaatgtatc atgaacggag
      661 tcaaattcca ccaggcaaaa gttattaaag tgattcacga agaaagcaaa agcatgctga
      721 tttgtaatga cggcatcacc attcaggcga ctgtcgtatt agatgcgaca ggctttagcc
      781 gctctcttgt acaatatgat aagccatata acccaggcta tcaagttgca tatggcatcc
      841 ttgcagaggt ggaagaacac ccatttgacg ttaataaaat ggtttttatg gattggcgtg
      901 acagccactt aaagaacaat accgatctga aagagcgtaa tagtcgcatt ccgactttcc
      961 tctatgccat gccgtttagt agcaaccgca tttttctgga ggaaacaagc ctggtcgccc
     1021 gtccgggtct tcgcatcgat gacattcagg aacgcatggt ggcccgcctg aaccatctgg
     1081 gcatcaaagt caaaagtatt gaagaagatg aacactgcct gattccgatg ggcggtcccc
     1141 tgccggtgct tccgcaacgc gtcgtgggca ttggcgggac tgcgggcatg gttcaccctt
     1201 cgaccggata tatggtggcg cggacccttg ctgccgcccc tgtggtggct aacgctatta
     1261 ttcaatacct gggcagtgaa cgtagtcact ccggtaatga actgtcaact gcagtctgga
     1321 aggatctgtg gccaattgag cgccgtcgcc agcgtgaatt tttttgcttc ggtatggata
     1381 ttctgctgaa actggatctg ccggctaccc gtcgcttctt cgatgctttt ttcgatcttg
     1441 agccccgcta ttggcatggc tttctgtcca gccgtttatt cctgccggaa ctcatcgttt
     1501 tcggtttgtc cttgttttca catgccagca acacttctcg ttttgaaatc atgacgaaag
     1561 ggacggtacc tctggttaat atgattaaca acttgttgca agataaagaa taatgaagag
     1621 cgtaagacct ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1681 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcccgaaag
     1741 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     1801 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1861 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1921 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1981 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2041 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2101 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2161 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2221 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2281 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2341 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2401 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2461 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2521 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2581 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2641 gagcgtgggt cccgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2701 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2761 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgccttg
//
