LOCUS       sequence-229111-        3853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crtI in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::crtI
FEATURES             Location/Qualifiers
     source          1..3853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      123..217
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1909..1995)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2008..2026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2094..2198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2199..2267,2268..3059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2417..2436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3230..3818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3719..3738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
       61 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcct
      121 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
      181 gctcggttgc cgccgggcgt tttttattgg tgagaatcca gtgtgctctt cgatgacatc
      241 agctctcccc cgcccggcac caagtccgta cgcacgccgt aaaacggcgt tggttattgg
      301 cgcaggtttt ggtgggttgg ccctgggcat tcgtctacag tcgttaggtt ttgatacaac
      361 aattttggaa cgtctggatg gtcctggtgg tcgcgcgtat caaaaacgta ccccagatgg
      421 ctatgtcttt gacatgggtc cgactgtgct gacggtgccg cattttatcg aagaactgtt
      481 tgcgcttgaa cgtgatcgtg ccggcctgga tgcccccgat tatcctcctg aagtgttgtc
      541 gggcgagcgc gttaaggaag gcgtttctgg tggcccgcat acgagccggt atgtcacctt
      601 agtgccgatt ctgccctttt accgcattgt ttttcacgat ggcacgtatt ttgattatga
      661 tggcgaccct gaaagtactc ggcggcagat tgctgaattg gcccctggcg acttagccgg
      721 gtatgaacgc tttcatgccg atgccgaggc catctttcgt cggggcttcc tggaactggg
      781 ctacacgcac tttggtgacg tcccgacgat gctgcgggtt gtacctgatc tgctcaaact
      841 ggacgccgtt cggaccctgt tctcctttac gagtaagtac tttcagagcg acaaactgcg
      901 ccaagtgttc tcttttgaaa cccttttggt gggtgggaat cctctgagtg tgccggcgat
      961 ctatgcaatg attcacttcg ttgaaaagac ttgggggatc cactatgcca tgggcggcac
     1021 aggcgcactg gtgcgcggcc tagtccaaaa atttgaggag ctgggtggcg ccattcgtta
     1081 tggcgccggc gtcgatgaag tactggtgga tggcaatctg cctggtaaac gcacagcgcg
     1141 gggtgtgcgc ctggaaagcg gcgaagaact gcgcgccgac ctggtggcgt ccaatggcga
     1201 ttgggctaac acgtatctga aacgcgtccg gccatcggca cgtctggtca actctgattt
     1261 acgcgtgaaa gccgcatctg aaagtatgag cctgctcgtg gtttatttcg ggtttcgcgg
     1321 cggtgatgac ctgcccctca aacatcataa tattttatta ggcccacgct acgaggctct
     1381 gctgagcgaa atctttggca caaaacggtt gggcgaagat tttagccagt acctgcacgt
     1441 cccaacgctc accgatccgg ctctggcacc cgcgggtcat catgcggcct atacacttgt
     1501 cccggtgccg cataatggct cgggtattga ttgggacgtg gaaggtccaa agcttgccga
     1561 agcagccctg gcagatatcg agcgccgcgg tttgattccg ggcctccgtg aacggctcac
     1621 acattttgaa tttattacgc cagattattt cgcaggcact ctcgattcct atctggggaa
     1681 cgcgtttggt ccggagccgc gtctggtcca gtcggcattt ttccgcccgc acaaccgcag
     1741 cgaggatctc cacaactttt acttagtcgg ggcgggcgcg cagccaggcg caggcacacc
     1801 gagcgttatg atgtccgcga aaatgacagc gcgcctaatc gctgaagatt tcggtatcca
     1861 tgctgatatc cggcgctaat gaagagcgta agacctctag ggcggcggat ttgtcctact
     1921 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
     1981 ctttcgtttt atttgatgcc cgaaagggcc tcgtgatacg cctattttta taggttaatg
     2041 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2881 ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtatcatt gcagcactgg
     2941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3841 tacggttcct ggc
//
