LOCUS       sequence-229112-        2299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator ECK120019600 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::ECK120019600
FEATURES             Location/Qualifiers
     source          1..2299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(291..309)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        377..481
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(482..550,551..1342)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(700..719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1513..2101
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2002..2021
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      join(2259..2299,1..54)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccagtg
       61 tggctcttca taattcagcc aaaaaactta agaccgccgg tcttgtccac taccttgcag
      121 taatgcggtg gacaggatcg gcggttttct tttctcttct caaggacgaa gagcgacctc
      181 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcccgaaagg gcctcgtgat
      301 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      781 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      841 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      901 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      961 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1021 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1081 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1141 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1201 ccgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1261 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1321 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1381 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1441 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1501 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1561 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1621 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1681 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1741 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1801 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1861 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1921 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1981 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2041 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2101 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2161 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2221 tgataccgct cgccgcagcc gaacgaccga gcgccttgga ctcctgttga tagatccagt
     2281 aatgacctca gaactccat
//
