LOCUS       sequence-229114-        2266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Terminator ECK120033737 in pStA0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Taylor GM, Mordaka PM, Heap JT
  TITLE     Start-Stop Assembly: a functionally scarless DNA assembly system 
            optimized for metabolic engineering.
  JOURNAL   Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 
            10.1093/nar/gky1182.
  PUBMED    30462270
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182."
COMMENT     Sequence Label: pStA0::ECK120033737
FEATURES             Location/Qualifiers
     source          1..2266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(54..140)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(153..171)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        239..343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(344..412,413..1204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(562..581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1375..1963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1864..1883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2121..2215
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gcgggctttt tttttcgacc aaaggggacg aagagcgacc tctagggcgg cggatttgtc
       61 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
      121 tgagcctttc gttttatttg atgcccgaaa gggcctcgtg atacgcctat ttttataggt
      181 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      241 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      301 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      361 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      421 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      481 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      541 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      601 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      661 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      721 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      781 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      841 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      901 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      961 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1021 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tcccgcggta tcattgcagc
     1081 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1141 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1201 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1261 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1321 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1381 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1441 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1501 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1561 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1621 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1681 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1741 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1801 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1861 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1921 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1981 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2041 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2101 ccgaacgacc gagcgccttg gactcctgtt gatagatcca gtaatgacct cagaactcca
     2161 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccagt
     2221 gtggctcttc ataaggaaac acagaaaaaa gcccgcacct gacagt
//
