LOCUS       sequence-229115-        6793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: pGL4.23-MYC-e3
FEATURES             Location/Qualifiers
     source          1..6793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    151..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1920..1939
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    2478..2526
                     /note="synthetic polyadenylation signal"
     misc_feature    2540..2631
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2580..2599
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2905..2914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2911..4563
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2977..2995)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4607..4728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4976..4993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5147..5735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5227..5246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5935..6726,6727..6793,1..2))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atactcgtcc tttttcaata ttattgaagc atttatcagg gttacactgc ggctcctcat
       61 cgcgataata aaccccagta aatgctttta aaaaacctcc cacacctccc cctgaacctg
      121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      241 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtggaaaacg tgtccccttg
      301 gtttcctctt tcagcattgt catttcttct gaaaggtgag cagctatgaa tctgagcaag
      361 agtgagaagg ttcgagctaa cacttgctta agagggctgg aactacatct gaaatagttt
      421 ggggtctgta gcttcttgtt agtccccaga atccctataa cagggatgct ctaagactgt
      481 ggtcctcaaa ctccactgca taatagaatc acctggggag atgttataaa tccaaatgcc
      541 caggtcccat ttcagacaaa atcaaaatct ttgcagctgg ggtactagca gcaggattta
      601 aaaaatcctc taggcaattc caagcttcaa cccagtctta gaacaactga tctaagagtc
      661 aaatccaaca ggaaaggaaa acatggcaga gaggaaagca tccccatcct tgagcagtgt
      721 catctgcaga actacttcag ctcattatga agccgctcct cattacgcct tacagggcag
      781 ctcggttgtc acaaggagct aacagacttg aaagttgccc tggatcactg cttttgtaac
      841 acacccttcc aaagtcttct ggaagcaggt aagggtagaa aataccaaca aatacagcca
      901 tgtgggtaac tcttgtggaa aaagcattgg gttgcgtgca gacagctggt tctggcccta
      961 ggctctttca ctaatactac gtaacttaaa aagcatataa cacttatgca tcagtttctt
     1021 cagtagtgaa ataaagataa gaactgactt acccattaga taagatgttg tggggtacaa
     1081 gccagaagac ataggtcagc tgtggcaaag catttttatt taacatgttt tccaggtctt
     1141 caatggaaca taaaaagttt aggagagaga aatgtcccct taaacatttt ttttcaatga
     1201 gagaagggag gagtcaagga gtagagttgg tcggccacat gaaagctccc ttcagagcag
     1261 gctgccgtgg tgcctgccca caaggggtac aagtggggcc agtgataggg caggagctca
     1321 gggtgtgtcc aagggctgcc ccagcaaaga cgttaagata ggatatgtta tctgggaaca
     1381 atggaacaac tgtaccgttg tcctaaacct gaatctgaca agaggggata agactccagg
     1441 aagcatgtcc ccaaaccact ggccagagtg gccccaacaa accactggtc catttgttca
     1501 ttcctttatc tacttactca ttcatgtacc ttacagtagt gtatgtcaac tacatatcaa
     1561 gaattgtatg gatgtacaag attgtaggaa tgctggaatg aataaggctc actccgcacc
     1621 ctcaggaggg acaaagataa gtagggcaaa caagtggtat ttaagtacaa cacaatgtgc
     1681 taaagtataa taaaataaca agtataatag taaatagcca ccactcattg atggccaact
     1741 atgcacaggc accatacttg gtgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     1801 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     1861 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     1921 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     1981 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     2041 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     2101 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     2161 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     2221 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     2281 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgaattt tgttaaaatt
     2341 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     2401 cccttataaa tcaaaagaat acgtctctca aggataagta agtaatatta aggtacggga
     2461 ggtattggac aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     2521 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     2581 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tggcctaact
     2641 ggccggtacc tgagctcgct agcctcgagg atatcaagat ctggcctcgg cggccaagct
     2701 tacccccgag ctgtgctgct cgcggccgcc accgccgggc cccggccgtc cctggctccc
     2761 ctcctgcctc gagaagggca gggcttctca gaggcttggc gggaaaaaga acggagggag
     2821 ggatcgcgct gagtataaaa gccggttttc ggggctttat ctaactcgct gtagtaattc
     2881 cggcaatccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc
     2941 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
     3001 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
     3061 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
     3121 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
     3181 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
     3241 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
     3301 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
     3361 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
     3421 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
     3481 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
     3541 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
     3601 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
     3661 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
     3721 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
     3781 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
     3841 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
     3901 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
     3961 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
     4021 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
     4081 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
     4141 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
     4201 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
     4261 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
     4321 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
     4381 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
     4441 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
     4501 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg
     4561 taataattct agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat
     4621 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4681 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4741 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     4801 aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt
     4861 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     4921 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
     4981 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5041 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5101 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5161 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5221 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5281 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5341 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5401 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5461 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5521 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5581 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5641 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5701 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5761 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5821 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5881 tatatgagta aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca
     5941 gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc
     6001 ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc
     6061 agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc
     6121 agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat
     6181 gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt
     6241 ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc
     6301 tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag
     6361 ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt
     6421 aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac
     6481 cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg
     6541 cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat
     6601 cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc
     6661 gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc
     6721 ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa
     6781 atgttggatg ctc
//
