LOCUS       sequence-229119-        7438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: pGL4.23-MYC-e4
FEATURES             Location/Qualifiers
     source          1..7438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    151..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2565..2584
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    3123..3171
                     /note="synthetic polyadenylation signal"
     misc_feature    3185..3276
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3225..3244
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      3550..3559
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3556..5208
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3622..3640)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5252..5373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5621..5638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5792..6380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5872..5891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6580..7371,7372..7438,1..2))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atactcgtcc tttttcaata ttattgaagc atttatcagg gttacactgc ggctcctcat
       61 cgcgataata aaccccagta aatgctttta aaaaacctcc cacacctccc cctgaacctg
      121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      241 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtggaaaacg ggacagggac
      301 atggtcagaa ctgtgctttc agaatttgga cttggccact ggaagaagag acagttaaac
      361 tcaagttgcc caacatgagg catgggatgg gagaggttgg ggaagggatg gaaggaaaca
      421 gagtgggcac caacaggaag gaagcaaagt caggaactgc tggctggaat gggtccaggg
      481 agagggaaaa ccacttctcc ttcctcttct tatgattgca agattagtac ttgagaatta
      541 ggctgcacag gccaggactt cccaaaactc aggagggaaa gagcccagag cacgtgtctc
      601 caatggaggc tatagttcca aatcacgtga tcatcggtca tctccttgcc caccctgaag
      661 cccttgtccc cagtgtactt tgggctcaga cagtccctca ggggatacag cagtgtcctt
      721 tccttcagca ttcagggcaa atgagaggca aagagatcaa tggaatgcaa aagcgttact
      781 atagatgaga agtcggacgc acagagaggt tccaccactt gcctaatatc acacagcaag
      841 tacatgccag aatcaaaatt caaacctaga gccccggtgt aaaccttacc tgaatcagtc
      901 agggagcagc agggaggcag tggaaggata ggccccctga cctcactccc cgcctgcctt
      961 cccatcctgc cagtgccttc caaactgata ccagagagca tggcagccct gtgttgcatc
     1021 caccctgata atgcatccac acaggtcagc cttctgggca caaaacaagg cagagaagag
     1081 caaggagtgg acctggaggg gcccacggaa gctccccagc acccaccact ccaatggccc
     1141 agcccctggt aggtctccag tgcccttcac cctacagagc cacagaatcc ccagaccttg
     1201 acgctgcagc tagggcccgc ctgcctctca agagaagggc agggccccac acaggcaagt
     1261 gctctgcctc cgcgcaaagc tgggcatgcg tcatcacact cccgccttcc ttcctcattc
     1321 actctccctt gccctgcttc tatcagtggc tccaagacgc aaacatctcg aaatgacctg
     1381 cttctgtgta ggtcagctaa cagaagtgct gtcttaagaa atgagggcca gctgatgccc
     1441 actaccagac ccagccagcc cctcccagcc tccctgccag ccctgcacat ggaggcctgg
     1501 cggccaggaa aaagaaaggg ctcctatctc tgattcctca gggcctctga taagaaatgt
     1561 catcccttgc tgggtaatat taccaagcca gataaaaatc tctatcaagc ttaaacatat
     1621 ggctggctgc agaacacaga gtccagtgga gaagggtaat ttatttgctt aagctgaaaa
     1681 ccctgggcag ccgaaagaag tgcctgaata aaagagtaag agccaaatta taacctcttc
     1741 tgacttggca tggggatttt tccctgggaa ggatgagaca cctgagaaca acagggggct
     1801 ctccacacat ttctcctgac acaataagcg ctttcgcctt cctctttctt caaagggcac
     1861 tttcacttat gctatcacat ttaatcctca ctacaacctc aagagaggca tttttagctt
     1921 cctttacaga tcaggaaatt gagattcagc aaggttaagt aaagtggcca aggtcacaca
     1981 ggcagccatg ctggagcgag gacccaaacc ccagcaggtc tgactcacca tcagatgggg
     2041 aagctgatcc cagcttctgg ggaggatcgg ctcatacatc cacttaatca gcgaagtcag
     2101 atctgcccaa cttaactgac aatgtcagct acacattgta atcttgaagg tccatttcaa
     2161 ggctctcccg tctccttgtt tctctgtagc ctaaaggata atgagaaagc cttgcagagc
     2221 acagaagctc aaggaaaagg acgggaggcc agggatagta cccaggagag cagtgagtga
     2281 cctcagggct tagattccag cctcatatgc tcaaggagta gcaggtagaa ggaaactgtg
     2341 gaaagaaaac tcctctctct gttcattcat tcattcacaa atattagtgg aaagcctatt
     2401 gtgtgccgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca
     2461 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     2521 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     2581 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     2641 gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg
     2701 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
     2761 cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg
     2821 taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag
     2881 cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt
     2941 tccgcttcct ttagcagccc ttgcgccctg aattttgtta aaattcgcgt taaatttttg
     3001 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3061 agaatacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc
     3121 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     3181 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     3241 tccccagtgc aagtgcaggt gccagaacat ttctctggcc taactggccg gtacctgagc
     3301 tcgctagcct cgaggatatc aagatctggc ctcggcggcc aagcttaccc ccgagctgtg
     3361 ctgctcgcgg ccgccaccgc cgggccccgg ccgtccctgg ctcccctcct gcctcgagaa
     3421 gggcagggct tctcagaggc ttggcgggaa aaagaacgga gggagggatc gcgctgagta
     3481 taaaagccgg ttttcggggc tttatctaac tcgctgtagt aattccggca atccggtact
     3541 gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     3601 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     3661 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     3721 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     3781 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     3841 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     3901 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     3961 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     4021 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     4081 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     4141 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     4201 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     4261 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     4321 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     4381 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     4441 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     4501 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     4561 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     4621 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     4681 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     4741 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     4801 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     4861 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     4921 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     4981 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     5041 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     5101 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     5161 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaata attctagagt
     5221 cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     5281 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5341 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     5401 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     5461 ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     5521 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     5581 gcaactcgta ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc
     5641 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5701 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5761 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5821 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5881 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5941 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6001 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6061 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6121 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6181 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6241 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6301 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6541 ggtctgacag cggccgcaaa tgctaaacca ctgcagtggt taccagtgct tgatcagtga
     6601 ggcaccgatc tcagcgatct gcctatttcg ttcgtccata gtggcctgac tccccgtcgt
     6661 gtagatcact acgattcgtg agggcttacc atcaggcccc agcgcagcaa tgatgccgcg
     6721 agagccgcgt tcaccggccc ccgatttgtc agcaatgaac cagccagcag ggagggccga
     6781 gcgaagaagt ggtcctgcta ctttgtccgc ctccatccag tctatgagct gctgtcgtga
     6841 tgctagagta agaagttcgc cagtgagtag tttccgaaga gttgtggcca ttgctactgg
     6901 catcgtggta tcacgctcgt cgttcggtat ggcttcgttc aactctggtt cccagcggtc
     6961 aagccgggtc acatgatcac ccatattatg aagaaatgca gtcagctcct tagggcctcc
     7021 gatcgttgtc agaagtaagt tggccgcggt gttgtcgctc atggtaatgg cagcactaca
     7081 caattctctt accgtcatgc catccgtaag atgcttttcc gtgaccggcg agtactcaac
     7141 caagtcgttt tgtgagtagt gtatacggcg accaagctgc tcttgcccgg cgtctatacg
     7201 ggacaacacc gcgccacata gcagtacttt gaaagtgctc atcatcggga atcgttcttc
     7261 ggggcggaaa gactcaagga tcttgccgct attgagatcc agttcgatat agcccactct
     7321 tgcacccagt tgatcttcag catcttttac tttcaccagc gtttcggggt gtgcaaaaac
     7381 aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt ggatgctc
//
