LOCUS       sequence-22912-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V8 BB.GFPLT CS2+
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     609..629
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(720..739)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(721..739)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
       61 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
      121 ccatggccaa cacttgtcac tactctcact tatggtgttc aatgcttttc aagataccca
      181 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
      241 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
      301 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      361 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      421 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
      481 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
      541 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
      601 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
      661 ctatacaaag gatcccatcg attcgaattc aaggcctctc gagcctctag attctgcagc
      721 cctatagtga gtcgtattac gtagatccag acatgataag atacattgat gagtttg
//
