LOCUS       sequence-229123-        7265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Fulco CP, Munschauer M, Anyoha R, Munson G, Grossman SR, Perez EM, 
            Kane M, Cleary B, Lander ES, Engreitz JM
  TITLE     Systematic mapping of functional enhancer-promoter connections with 
            CRISPR interference.
  JOURNAL   Science. 2016 Sep 29. pii: aag2445.
  PUBMED    27708057
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Sep 29. pii: aag2445."
COMMENT     Sequence Label: pGL4.23-MYC-e5
FEATURES             Location/Qualifiers
     source          1..7265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    151..272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2392..2411
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    2950..2998
                     /note="synthetic polyadenylation signal"
     misc_feature    3012..3103
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3052..3071
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      3377..3386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3383..5035
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3449..3467)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5079..5200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5448..5465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5619..6207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5699..5718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6407..7198,7199..7265,1..2))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atactcgtcc tttttcaata ttattgaagc atttatcagg gttacactgc ggctcctcat
       61 cgcgataata aaccccagta aatgctttta aaaaacctcc cacacctccc cctgaacctg
      121 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
      181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
      241 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtggaaaacg agtggggaca
      301 tgattccaaa tacatgggct gccttcaaat cctggcccac aagcctacat ccttacaaca
      361 gaaccctctc tggaaatgag acaattattt ctttctagtg ggggttgcag agagtagagg
      421 aagagagttg gaaaggagaa ggagagctag tggatgggaa accaacaaaa tgtggttcat
      481 tttcaggaag ctgcgaggct ggatctttca gaacagctca tattttttcg agatgcattt
      541 tttttgaggt cccccactcc ggatacccca gtgccctcct ttcagccttg gcccttgcat
      601 caccaccagc caggggaagg agccgtggca tggcagtggt cacagttctc ttggataagc
      661 tttaccgtaa tcttcataaa cctcaatgct caaggtgcag agggagattg gaggaaactt
      721 gtttttccgt tggtttctct ctgactgctg cggctaatca ttcctagaac aggaagctgg
      781 agaaatgtag gtaggagaca ctcttctgac aagtcaggtt gcctcaccct tccagggcac
      841 aactgattag gaaaagcttc agggttacat aacctccttc ctttacggtg gttttccatc
      901 aaaaggtgaa tggactcagc cctgagaact gcccacccct aagaagcggg gaagggatgt
      961 cttggttgta ttttagtatt gctgcctcct tcatcacaat gccccagtgc acaagtaagg
     1021 ggaggaaaat aggatgcatt aatttattcc atgaaccttt attgagtgtc cattcttcta
     1081 tgtatttgaa atacatcagt aaaggaaatg aactctaccc tggtacactc atatcctagt
     1141 ggagggacac agagtaaacc ataatacata agtaatttac attgtgggtt agaagttgat
     1201 gaatgaaatc aagaaaagta gagcagggac agagggatag gtggttggga gggcaaaggc
     1261 atggattgca atttttaacg gagcacaagg taagactcct tgaaaagatg acatttgagc
     1321 aaagacttga aagagatgaa ggtgtgagct acatgactat atgaagaaat agcaaaggcc
     1381 taaggcagga gtgtttccag agttgttgtc agggtgggag gagcacagtg agcaaggaag
     1441 aaaaaagtaa aagatgatgt atgtgattgg ctggggggca ggtgtcagat caggtggcac
     1501 tttgcaagtg attgttaagg actctgtgct tttagagtaa ataaaatggc agctattatc
     1561 gttctgagaa gagtaacaga accagcacgt tcaccctggt gctccttctg gggtgagagc
     1621 aaacacagaa agggtcatcc gatatcatta acagctgtct gtactggagg cctctttagg
     1681 cttctgggtc aagtcaaata acctacagac ccacctatcc tcatggccgg ggtgggggtg
     1741 ggccagaaga gtgacagcct atgtgtttaa caacatgatt taaatcattc aagtgaccca
     1801 ctgagagctg tgaaagcaat tttgaatttc ttttcctgca gaaggaatgg ctttacctta
     1861 agtcactacc ttcctcaatc ttctttgtga ccaaaaagac agattccaaa cactgccagc
     1921 tgagtacaat ctacaccctc caaatatagc agagctaact gcctacaccg gaccctggtg
     1981 ccattagctc atgctctagc tccctgcact ggccacccat actgctttat gaaatgcaat
     2041 ctctttttaa agataatgac acagtaagtg tgttatgact tcagtgatac agtaagttag
     2101 gcaaattctt tgaaagctat cagctcattt tgagcagggc caccagcctc tggtagagag
     2161 aagtttgttg atctgaagtc ataaaaccaa ggggtaaagg acatgctgaa ccagacgata
     2221 aacaccgtca tggcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg
     2281 tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc
     2341 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca
     2401 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca
     2461 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
     2521 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
     2581 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
     2641 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
     2701 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
     2761 tacgtgttcc gcttccttta gcagcccttg cgccctgaat tttgttaaaa ttcgcgttaa
     2821 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     2881 aatcaaaaga atacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
     2941 acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     3001 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     3061 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa ctggccggta
     3121 cctgagctcg ctagcctcga ggatatcaag atctggcctc ggcggccaag cttacccccg
     3181 agctgtgctg ctcgcggccg ccaccgccgg gccccggccg tccctggctc ccctcctgcc
     3241 tcgagaaggg cagggcttct cagaggcttg gcgggaaaaa gaacggaggg agggatcgcg
     3301 ctgagtataa aagccggttt tcggggcttt atctaactcg ctgtagtaat tccggcaatc
     3361 cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     3421 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     3481 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     3541 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     3601 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     3661 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     3721 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     3781 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     3841 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     3901 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     3961 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     4021 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     4081 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4141 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     4201 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     4261 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4321 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     4381 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     4441 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     4501 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     4561 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     4621 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     4681 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     4741 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     4801 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     4861 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     4921 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     4981 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaataatt
     5041 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     5101 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     5161 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     5221 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     5281 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
     5341 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     5401 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     5461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6361 taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga
     6421 tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc
     6481 ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga
     6541 tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga
     6601 gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct
     6661 gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg
     6721 ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc
     6781 agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag
     6841 ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag
     6901 cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt
     6961 actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt
     7021 ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc
     7081 gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc
     7141 ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg
     7201 caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga
     7261 tgctc
//
