LOCUS       sequence-229133-        6876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_195190-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2040..2057
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2109..2164
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2163..2182)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4400..4418)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4486..4590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4591..4659,4660..5451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4809..4828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5622..6210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6111..6130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6364..6381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6498..6519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6534..6539,6540..6557,6558..6564)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6572..6588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6577..6599
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6596..6612
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6596..6612
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 aacctgagcc tgaaccagag cccgtgcccg tgtcccggaa cagcaaggac gtgtccatcc
     1261 agaccgacgt ggacctggac ccccggttcc tgctgctgga cctgaagaga gagatcggcc
     1321 ggctgaagga caccttcaac gccctggtgg ccaagatcga caccatcccc cccagcagca
     1381 ttgccacagg cgcgccgtcg acctccatca cggcctataa gagtgagggg gagtcagcgg
     1441 agttctcctt cccactcaac cttggagagg aaagcctgca gggagagttg agatggaagg
     1501 cagagaaggc tccttcttcc cagtcctgga tcaccttctc cctaaagaac caaaaggtgt
     1561 ctgtgcagaa gtctactagc aaccccaagt tccagctgtc cgaaacgctc ccactcaccc
     1621 ttcagatacc ccaggtctcc cttcagtttg ctggttctgg caacctgacc ctgactctgg
     1681 acagagggat actgtatcag gaagtgaacc tggtggtgat gaaagtgact cagcccgaca
     1741 gcaacacttt gacctgtgag gtgatgggac ccacctcacc caagatgaga ctgatcttga
     1801 agcaggagaa tcaggaggcc agggtctcca ggcaggagaa agtgattcaa gtgcaggccc
     1861 ctgaagcagg ggtgtggcaa tgtctactga gtgaaggtga agaggtcaag atggactcca
     1921 agatccaggt tttatccaaa gggttgaatt ccggcagcct gcaccacatc ctggacgccc
     1981 agaagatgct gtggaaccac agggacagga acctgcctcc tctggcccct ctgggccccc
     2041 accaccatca ccaccattga gtctagacac gtgattaatt aaggatcccc cgacctcgac
     2101 ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2161 ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc ggagatctct
     2221 agctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     2281 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     2341 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     2401 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     2461 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     2521 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     2581 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     2641 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     2701 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     2761 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     2821 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     2881 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     2941 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     3001 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     3061 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     3121 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     3181 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     3241 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     3301 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     3361 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     3421 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     3481 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     3541 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     3601 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     3661 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     3721 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     3781 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     3841 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     3901 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     3961 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     4021 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     4081 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     4141 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     4201 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     4261 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     4321 atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca aacatgagaa
     4381 ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4441 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4501 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4561 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4621 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4681 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4741 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4801 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     4861 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4921 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4981 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5041 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5101 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     5161 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5221 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5281 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5341 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5401 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5461 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5521 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5761 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6241 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6301 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6361 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6421 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6481 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6541 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6601 aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt ctcatgtttg
     6661 acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt
     6721 aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
     6781 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
     6841 tgtacattta tattggctca tgtccaatat gaccgc
//
