LOCUS       sequence-229138-        8415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_194920-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3579..3596
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3648..3703
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3702..3721)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5939..5957)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6025..6129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6130..6198,6199..6990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6348..6367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7161..7749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7650..7669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7903..7920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8037..8058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8073..8078,8079..8096,8097..8103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8111..8127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8116..8138
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8135..8151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8135..8151
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 acaagaacat catcctgaac aacaacccca gcgacttcta ccagcagctg aacgcctggc
     1261 ccgacttcca gctggccgcc ttcaccgacc tgaacgccga ccggcggatg gacatcatct
     1321 tcgccaacgg cgacggcagc aagctgttcg ccgtgctgca gagcgacaac aagggcttcc
     1381 agagcaccag cagcatcctg agcagcgtga agttcagccc ccctgtggaa atcatcggca
     1441 acgtgctgcc caagcccgtg cggggcgtgg ccaccagcga tttcaacggc gacagccgga
     1501 tcgacctgct gctggtcaca ggcagcagcg acggcggacc cttcgacgtg tacgtggcct
     1561 acggcctgag cggcgagaac cggggcaagt tcgacagccc caagttcctg gacaccttca
     1621 ccagccagcc cctcgtgtgc gacctgaaca gcgacatgat cgccgatgtg tacggcgagg
     1681 tgtccggcca cagagtggcc tacatcggcg gcaacaagct gaccaagctg agcgccacct
     1741 acagcggccc cagctggtcc agactgggct acagcgcctt cggcgacatc aatggcgacg
     1801 gcgtgcccga catcgtgatc ctggtggaca acgagaaccg gcagcagctg caggtcatcc
     1861 tgcggaccca gagcagcatc ggcagcctgc ccgatgtggc caacgccgag ctgatcgacc
     1921 tggaccccaa gctgctgagc accccccaga gcgtgctggg cctgttcgtg ctgaacgact
     1981 tcgactacga cggcctgatt gatctgctgc tgcccgtgtg ccgggacgcc aagtgcctgg
     2041 ataccagctc catcaacatg tacagcttcc gggaccggaa gtggtccccc gtgaacgtga
     2101 tctgggagcc cagcaacgtg aaagaaaaca ccatgcggtg gtccctgacc agcacccctc
     2161 agatcaagag cggcctgctg accagctccc tgatcggacc cgctgtgggc gacgtgaaca
     2221 tggacggcaa gcccgatctg ggcctgggcg tgacctcttg gagcgagaac ggccagaaca
     2281 tgggcgtgta ccccggcgtg ttcgtgaaca agggcctgaa cagcaacggc atcctgacct
     2341 tccagctgac cctggtgccc ggcgtgcagc tgccccccga caacagcaga ctgcggcagc
     2401 tggccttctt cgaccagagc gacgactcca tcctggattt cttcatcgtg tccctgaaca
     2461 gccacgacca gcccagcgcc cagctgttcc tgagcaccgt ggataccgac ccctacttcc
     2521 tgaaagtgac cgtgctgaat ggcctgtgca gcagcgccga cagctgctcc gacggcaagc
     2581 tgccttacgg cctgaccgtg cccggcatca gcagcaacct gaataccgag gccgctgccg
     2641 gcggaaggca tctgtctgcc gctctgctga gcagccagag ctgctgcagt gccctgcagg
     2701 tccccttcgc catcttcggc ctgggccagt tcgccaacta cgtggaaaag ctgaccatca
     2761 gcatcccccc cagcaaagag ctgatcagaa gccggctgct gagcttcatc gtgcccaagg
     2821 cccagattgt gatcaacccc taccccctgg acaaccctag cgcctggacc atgaagctgt
     2881 ttctgcagcc cctgtacaac atgaaggtgc tgtatatcgc catcactggc gcgccgtcga
     2941 cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc ccactcaacc
     3001 ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct ccttcttccc
     3061 agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag tctactagca
     3121 accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc caggtctccc
     3181 ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata ctgtatcagg
     3241 aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg acctgtgagg
     3301 tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat caggaggcca
     3361 gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg gtgtggcaat
     3421 gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt ttatccaaag
     3481 ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg tggaaccaca
     3541 gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac caccattgag
     3601 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     3661 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     3721 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     3781 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     3841 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     3901 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     3961 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     4021 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     4081 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     4141 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     4201 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     4261 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     4321 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     4381 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     4441 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     4501 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     4561 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     4621 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     4681 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     4741 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     4801 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     4861 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     4921 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     4981 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     5041 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     5101 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     5161 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     5221 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     5281 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     5341 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     5401 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     5461 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5521 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     5581 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     5641 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5701 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     5761 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     5821 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     5881 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     5941 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6001 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6061 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6121 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6181 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6241 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6301 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6361 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6421 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6481 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6541 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6601 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6661 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6721 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6781 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6841 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6901 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6961 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7021 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7081 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7141 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7741 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7801 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7861 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7921 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     8041 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8101 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     8161 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     8221 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     8281 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     8341 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     8401 gtccaatatg accgc
//
