LOCUS       sequence-22914-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V18 pCS3+ FLT
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..49
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    105..239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(129..148)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     183..202
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(352..372)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(352..370)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(391..407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(391..407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(404..426)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    415..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(439..445,446..463,464..469))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(622..639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tnntcttttg caggatccac catggnatta caaggatgac gacgataaga tctcgaattc
       61 aaggcctctc gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa
      121 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      181 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
      241 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
      301 aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg
      361 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      421 tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc
      481 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      541 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      601 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      661 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaa
//
