LOCUS       sequence-229143-        6912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_019350-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2076..2093
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2145..2200
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2199..2218)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4436..4454)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4522..4626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4627..4695,4696..5487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4845..4864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5658..6246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6147..6166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6400..6417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6534..6555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6570..6575,6576..6593,6594..6600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6608..6624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6613..6635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6632..6648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6632..6648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 acagatgcta cgtgtgcagc aagtgccagg accccttcag agtgaaggac accgagatcc
     1261 agaacggctg caccttctgc agcaccatcc ggacctacgt gcaggacaag ctgcaggtca
     1321 caagccggtc ctgcgtgccc gtgtgcgtgg aagccgatgc cagaagatcc ggcagcggca
     1381 tcgtgaccag ctgctgccag gacgacctgt gcaacagcgg cacaggcgcg ccgtcgacct
     1441 ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca ctcaaccttg
     1501 gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct tcttcccagt
     1561 cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct actagcaacc
     1621 ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag gtctcccttc
     1681 agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg tatcaggaag
     1741 tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc tgtgaggtga
     1801 tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag gaggccaggg
     1861 tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg tggcaatgtc
     1921 tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta tccaaagggt
     1981 tgaattccgg cagcctgcac cacatcctgg acgcccagaa gatgctgtgg aaccacaggg
     2041 acaggaacct gcctcctctg gcccctctgg gcccccacca ccatcaccac cattgagtct
     2101 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     2161 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     2221 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     2281 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     2341 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     2401 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     2461 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     2521 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     2581 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     2641 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     2701 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     2761 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     2821 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     2881 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     2941 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     3001 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     3061 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     3121 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     3181 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     3241 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     3301 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     3361 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     3421 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     3481 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     3541 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     3601 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     3661 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     3721 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     3781 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     3841 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     3901 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     3961 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4021 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     4081 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     4141 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     4201 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     4261 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     4321 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     4381 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     4441 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4501 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4561 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4621 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4681 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4741 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4801 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4861 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4921 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4981 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5041 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5101 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5161 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5221 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5281 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5341 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5401 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5461 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6421 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6481 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6541 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6601 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6661 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     6721 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
     6781 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
     6841 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
     6901 caatatgacc gc
//
