LOCUS       sequence-229144-        6975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_021220-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2139..2156
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2208..2263
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2262..2281)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4499..4517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4585..4689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4690..4758,4759..5550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4908..4927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5721..6309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6210..6229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6463..6480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6597..6618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6633..6638,6639..6656,6657..6663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6671..6687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6676..6698
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6695..6711
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6695..6711
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 aggaactgtc cgtgtctcct atcgcccagg ccctgagagt gcacgagagc ggcatcttcg
     1261 agtgcagctt cgagaacagc agctacaacc tggacctgcg gtggatcctg ttcgacggca
     1321 ccgtgctgct gaacggcaac accagcgagg acggccggtt catcaacgag gacggcatcc
     1381 tgcggatgac caacctgacc atccccgaca gcggcgagta caactgcgag aacctggaaa
     1441 tcaacaccac cgtgaccgcc cacctgaagg tgtacatcat gccctctggc gcgccgtcga
     1501 cctccatcac ggcctataag agtgaggggg agtcagcgga gttctccttc ccactcaacc
     1561 ttggagagga aagcctgcag ggagagttga gatggaaggc agagaaggct ccttcttccc
     1621 agtcctggat caccttctcc ctaaagaacc aaaaggtgtc tgtgcagaag tctactagca
     1681 accccaagtt ccagctgtcc gaaacgctcc cactcaccct tcagataccc caggtctccc
     1741 ttcagtttgc tggttctggc aacctgaccc tgactctgga cagagggata ctgtatcagg
     1801 aagtgaacct ggtggtgatg aaagtgactc agcccgacag caacactttg acctgtgagg
     1861 tgatgggacc cacctcaccc aagatgagac tgatcttgaa gcaggagaat caggaggcca
     1921 gggtctccag gcaggagaaa gtgattcaag tgcaggcccc tgaagcaggg gtgtggcaat
     1981 gtctactgag tgaaggtgaa gaggtcaaga tggactccaa gatccaggtt ttatccaaag
     2041 ggttgaattc cggcagcctg caccacatcc tggacgccca gaagatgctg tggaaccaca
     2101 gggacaggaa cctgcctcct ctggcccctc tgggccccca ccaccatcac caccattgag
     2161 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     2221 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2281 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     2341 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     2401 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     2461 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     2521 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     2581 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     2641 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     2701 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     2761 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     2821 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     2881 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     2941 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     3001 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     3061 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     3121 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     3181 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     3241 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     3301 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     3361 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     3421 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     3481 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     3541 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     3601 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     3661 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     3721 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     3781 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     3841 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     3901 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     3961 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4021 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4081 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     4141 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     4201 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4261 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4321 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     4381 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     4441 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     4501 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     4561 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4621 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4681 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4741 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4801 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4861 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4921 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4981 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5041 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5101 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5161 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5221 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5281 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5341 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5401 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5461 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5521 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5581 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5641 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5701 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5761 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5821 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5881 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5941 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6001 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6061 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6121 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6181 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6241 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6301 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6361 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6421 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6481 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6541 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6601 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6661 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6721 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     6781 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat
     6841 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     6901 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     6961 gtccaatatg accgc
//
