LOCUS       sequence-229152-        7464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_174580-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2628..2645
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2697..2752
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2751..2770)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4988..5006)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5074..5178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5179..5247,5248..6039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5397..5416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6210..6798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6699..6718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6952..6969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7086..7107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7122..7127,7128..7145,7146..7152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7160..7176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7165..7187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7184..7200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7184..7200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 gctaccacat ccgggacaac agcctggtgc ccccctacag cagcccccac tgggtgccat
     1261 acggcagcac cgtgatcgag ccccagttcg tgcggctgac cagcgacatc aagagcagcc
     1321 agggcggcat cttcaacatc aagcccctgt tcgcccggga ctgggaggtg gtcatcctgt
     1381 tccacgtgca cagccccaag accctcgtgg gcgacggctt cgccttctgg tacacccagc
     1441 acccccctag cagcggccct gccttcggca gcagagacaa gttccggggc ctggccgtgt
     1501 tcttcgacac ctacgccaac cagaacggcg agcacagcca cgagcacccc tacgtgtccg
     1561 ccatgatcaa cgacggcagc aagagctacg accacgacaa ggacggcacc ctgaccgagc
     1621 tgagcggctg cagcagcaac ttccggaaca acgactacag catggccacc atcagatacg
     1681 ccaacaacca gctgaaggtg tccctgaagt accagggcgc tgccgacccc gtggactgct
     1741 tcaccgtgga cggcgtgcgg ctgcccaccg gctactttat cggagtgtcc gccgccaccg
     1801 gcgacctgag cgacaaccac gacatctaca gcatccacac ctacgagctg gacgtgggcc
     1861 ggaaggacga ccccttcgcc gacttcagca agatcgagcc cagcgccgac caggaagccg
     1921 cccctagagc cagagtggac gacacccccc ccagccggtt cagcagagtg atcacaggcg
     1981 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2041 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2101 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2161 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2221 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2281 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2341 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2401 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2461 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     2521 tatccaaagg gttgaattcc ggcagcctgc accacatcct ggacgcccag aagatgctgt
     2581 ggaaccacag ggacaggaac ctgcctcctc tggcccctct gggcccccac caccatcacc
     2641 accattgagt ctagacacgt gattaattaa ggatcccccg acctcgacct ctggctaata
     2701 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     2761 gacatatggg agggcaaatc atttggtcga gatccctcgg agatctctag ctagaggatc
     2821 gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc ttcgcggggc
     2881 agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct gttccacatg
     2941 tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca tccttataaa
     3001 tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact ttgcagtctg
     3061 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     3121 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     3181 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     3241 ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt agtagtatat
     3301 actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc atatgctatc
     3361 gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc atgagctgtc
     3421 acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt tagtcacaag
     3481 ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg cctgcttctt
     3541 cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa ggtgtatgtg
     3601 aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga cggggggttc
     3661 agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg gcaataaata
     3721 ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg gggtggggac
     3781 aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca acacataatc
     3841 ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc aagacaggtg
     3901 aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc gacagcagcg
     3961 gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg ccaatggggc
     4021 ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc
     4081 agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat aacttggctg
     4141 attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac taatggcacc
     4201 ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat tgctgcgatc
     4261 tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt tggtcctcat
     4321 attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca tatactaccc
     4381 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4441 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4501 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4561 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctaat
     4621 agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata ctacccaaat
     4681 atctggatag catatgctat cctaatctat atctgggtag catatgctat cctaatctat
     4741 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4801 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     4861 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     4921 atccgggtag catatgctat cctcatgata agctgtcaaa catgagaatt ttcttgaaga
     4981 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5041 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5401 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5701 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     5761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6961 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7021 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7081 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7141 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7201 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
     7261 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
     7321 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     7381 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     7441 ttggctcatg tccaatatga ccgc
//
