LOCUS       sequence-229157-       10098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_129840-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..10098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5262..5279
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5331..5386
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5385..5404)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7622..7640)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7708..7812
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7813..7881,7882..8673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8031..8050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8844..9432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9333..9352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9586..9603
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9720..9741
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9756..9761,9762..9779,9780..9786)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9794..9810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9799..9821
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9818..9834
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9818..9834
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 gcatcagccc cgacatctac accgacccca acgagctggc cttcgaggtg tacagcagca
     1261 acaataccct ggaaaagtca ttcttcctga tccagcccac catggacggc agcatcattg
     1321 cccacctggg cagccacaac agcacccact tctacaagat ggcctctatc aacgtgttcc
     1381 tgaacaccca catcatcttc gtgtacaaga cccacagcag ccccctgtac gccctgtgca
     1441 gcgtggaaaa caactacctg atcgcccagc agaacacctt catcctgtgc gccttccggg
     1501 tgtacatcca gcccttcgac ctgcacgtgt tcagcaacag cagctgcacc atccggaccg
     1561 tgaacgagag cgagtgcgcc gccaagctgg acctggactt catcaagatg cagttccggg
     1621 cccacatgac cgagagcctg accatcctgg tcaagtacaa cgccaccgcc taccaggcca
     1681 actacaccga gccctacttc cactacgtgg aaagaagcga catcacccac agcctgatcc
     1741 accagagcca ggtgctgccc cccgtgaccg ccatccagaa acccgacttc gagtggtcca
     1801 acatcaacca cggcatcagc aacgcccccc tggtgcagat cggcatcagc tccctgcggc
     1861 tgaaccacga gttcagcctg cgggtggccg tgaacagaag cagccagatc cacgagttcc
     1921 ggctgggcat ccagttcggc cccaccgtca gattcgtgcg gcccaagccc agcaagttct
     1981 ggggcgtgaa agtgatcccc agccagtacg gcgtgaacat ccggttccgg aacaaaaagc
     2041 ccgacatgac ccagaagctg ggagcccaga tcaacgacaa gagcttcatg aacgccgccg
     2101 agctgatcat ggagtacatc ttccaggaaa accaccccct gggcagcagc ctgaacaacc
     2161 ggatcgacat caacagcatc gacgtgtcca gcatcgacag cggcagccgg tccagcatca
     2221 acgtgcccct gacccagttc aagagcgacc ggctgaacac caacaacctg cacggcctgg
     2281 acaacgaggt gttcctggac aacaccagcg tggacatcga gagcgacttc aacgaggaca
     2341 accagaacat ggaaaacaac aatctgggca tcaacaagca caacagcaac aacaacttca
     2401 tcgacaacaa cgactacagc ttcatcaagc agcccatcat ccaggtcaaa ctgatccact
     2461 tcatcaacca gagcaacctg agcaaagtga ccctgtcctc cctgaacaat ttcatcaacc
     2521 tggacatccc ccggctgctg atgcagagca acaacatgca catcttcgtg cagttcgagc
     2581 cccgggacat catcgagcct ccctggttcc ccgccatcag cagccccgag ccccggaagc
     2641 tgcggatcat cggcctgatc cctgagcagc acagcggcct gctgaacaac gacagaagct
     2701 acgagttctc cctgagcacc cactccgtgg gccggctggt ggacctgacc agaagcgtgt
     2761 tctgccggcc cagcaccacc agaggcctga gcgacaccct ggtggacgtg atcatcagca
     2821 gctcccccgc ctgcagcatc agcatgagac aggcccccag cgagatcgag aacatcagcc
     2881 cccccttcaa cgacggcttc ctgcgggacc tgaagctgag catgctgacc aaggtgtccg
     2941 gcggcttcgg catgcggacc ggctggaacg atcagctggt gctggccctg cccagctccg
     3001 tggaacagac ctaccggatc cccatcgtgc ccagcatcta cggccccatc agcctggaca
     3061 cccactggcc ctggcgggat tggagaggcc ctaccgtgcg gcggtggatg ctggaaggct
     3121 ttcagatcca caccaccaag cggctgctgc ggaagatcac cagctggaag atcccccaga
     3181 acagcgccct gaatagcaac aaccccgtga tccaccccca gaccgagaac gagtccttcg
     3241 ccggcatgga cagatacgag cagaccatgg tcaccgtgac agcccggatc tacaccatga
     3301 agcccggcac cggccagcgg atctacctgg gctccagctt tatcgacaag cagcagagca
     3361 gcagcagctt cagcatcccc cccatcagaa gcgagttcca caccaacgag gacggctctg
     3421 gccagctgat cttcgacatc accagactgc tgcctagctg ggccctgatc gtgaagcccg
     3481 tgaagtccca ccagagcagc aacttcgaca ccatcagcgc cgcctacctg taccgggaca
     3541 acttcagcgg cagaccttac ctgatcggcc ggtggcccgg caaggtggaa ctgagcctgg
     3601 tggccaccct gggcgacttc cagcctctgg ccacactgtc catcgacgtg gtggacttcg
     3661 acgacctgac cgtgcaccag cccaacagca tgtacaccaa tgagaacatc gacatcgtgg
     3721 ccctgaaggc cgagtgcatc gacccccacc tggaactgtc cggctccctg aatcccgcca
     3781 ccaccggcaa tagcgagggc ctgctgttcg acagctccag ccaggacccc taccggaaga
     3841 agtacagcca cctgttcgac gataagtacg acggcatgag aaccaccacc atccaggact
     3901 acagcctgcc caacccccac agactgaaca tcaatctgat cggcagatcc cccaagagcg
     3961 tgaagctggt caccgccgct gccagcagca ccatcagaca cccccagagc ttcaagttca
     4021 agctgatctg gcccgaccag aacacccccc ggatcatgga caccgtgggc cctagcgtgt
     4081 ccagcgccgt gtgccccatc catctgctgg tcatcaacat catcctgagc gacggctcca
     4141 tgatcggcgc ccacaccgtg cccaagaacc agctgagaat cagcagctac ggcagcaaat
     4201 acgtgtggga ccccgccatg agcgtgcagc ccatgaacga cgagagagag tacgcccaga
     4261 acatcaccgt gcctatcaac tccctgatca tctggcctgc cgtgctgacc gacaccaaag
     4321 agacaaagtt ccactccctg cccctggcca tcatggacga cgtgttcgtg tacatccgga
     4381 agtctggcat ggtgcaggac cccggcagcc cccacaaccc cagctactgg ttcagcaagc
     4441 agaagaagca caagaccagc gagaagatcc tgcggagcag cgagcaggaa aaggacagca
     4501 gcgtcgccag ctccaacacc ggcagctggc tgcagttcag cacctccttc agcctgagca
     4561 acaccgacaa gagcaacgac agcaagaacc agtacaccca gaccgtgtct ggcgcgccgt
     4621 cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc ttcccactca
     4681 accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag gctccttctt
     4741 cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag aagtctacta
     4801 gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata ccccaggtct
     4861 cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg atactgtatc
     4921 aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact ttgacctgtg
     4981 aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag aatcaggagg
     5041 ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc
     5101 aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag gttttatcca
     5161 aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc
     5221 acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat caccaccatt
     5281 gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct aataaaggaa
     5341 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     5401 tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag gatcgatccc
     5461 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     5521 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     5581 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     5641 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     5701 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     5761 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     5821 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     5881 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     5941 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     6001 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     6061 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     6121 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     6181 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     6241 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     6301 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     6361 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     6421 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     6481 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     6541 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     6601 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     6661 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     6721 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     6781 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     6841 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     6901 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     6961 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     7021 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     7081 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     7141 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     7201 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc taatagagat
     7261 tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc aaatatctgg
     7321 atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     7381 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     7441 gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg
     7501 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg
     7561 gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg aagacgaaag
     7621 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7681 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7741 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7801 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7921 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7981 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8041 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8101 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8221 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8341 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8401 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8521 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8641 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8701 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8761 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8821 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8881 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8941 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9001 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9061 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9121 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9181 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9241 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9301 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9361 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9421 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9481 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9541 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9601 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9661 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9721 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9781 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9841 tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
     9901 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
     9961 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
    10021 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
    10081 catgtccaat atgaccgc
//
