LOCUS       sequence-229158-        8865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_133270-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4029..4046
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4098..4153
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4152..4171)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6389..6407)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6475..6579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6580..6648,6649..7440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6798..6817)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7611..8199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8100..8119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8353..8370
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8487..8508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8523..8528,8529..8546,8547..8553)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8561..8577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8566..8588
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8585..8601
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8585..8601
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 aggacctgaa gcccaagaga aagagcggcg acgcccacat cagcatcacc caggacaaga
     1261 agaagatcaa gagctacatc gagcagttcg acgaccccga gacagtggaa aagttcctgc
     1321 gggacctgct gaaccccgac ctgatgttcc cccagatccc caaggacaaa gaagatgtga
     1381 acgtcgagag cgagagccag gacgaccccc tggacagcaa caccaaggac gagaaccagg
     1441 aactgaccga caagagcgag cacgacagca ccgccgacaa cgtgcccgcc agccagagca
     1501 tccccagcat caacgacgag aaaaccgtga agtccatcca cagcccccct gacaagcacg
     1561 aggacagcga gcctagcgtg gcccagcacc tgtacgacga ggccatgagc atcctggaaa
     1621 ccgagagcca caagaccagc gccgacaaga ccgccctgga actgctgagc gaggccagca
     1681 gacagggcca caccaaggcc cgggaacagg tggccatgct gaccctgctg ggcggctaca
     1741 ccgccgaccc tttccagagc gcctacagcg ccttcgagga actgagcaac gagggcaacc
     1801 ccagaggcca gttcggcctg ggctttctgt acgccagcgg cctgcacgtg aacgccagca
     1861 tcccccacgc cctgatctac ctgaccttca gcagcctggg cggcgacaac ttcgccgaca
     1921 tggccctggg ctaccggtac tggacaggcg tgggcgtgga agaggattgc gaggccgccc
     1981 tgacccacta ccacagagtg gcccaggtgg tgtacaaaga ggtgtccgag cggagcgaga
     2041 gcggcggacc tacactgctg ggccctatgg tgcgacgggc cagactgctg gacgagatcg
     2101 acgccatggg caacctggac ggcggcgacc tgaccatcag cgaggacctg ttccagtact
     2161 accagttcat ggcctacaag aaaaacgtgt ccgccatggt cggactgggc cagctgtact
     2221 actacggccg gcacggcgtg gaaatgaacc acgagaaggc cttttactac ttcaacctgg
     2281 ccgccgagag cggcagcgcc attgccatgg cctacctggg cgagatgtac atggtcggaa
     2341 gcaccgccgt gcccgccgac agcacaaagg ccctgaagta cctgcacaag agcgccgagg
     2401 aaaacaaccc catcggccag acaggcctgg ccctggccta tctgtatggc agagccggcc
     2461 tgcctgtgaa gcccgtgatc gccatggaac tgtttctgaa ggccgccgac cagggctggc
     2521 ctgaggctca gctgcacctg ggcagactgt ttctgggcac ccacggcatc aagaccgact
     2581 acaagtccgc cctgaaatac ttcaccatgg ccagccagca gggcaacgtg atcgccttct
     2641 accacctggc cgagatgcac gccaagggca ccggcgtgct gagaagctgt agcacagccg
     2701 ccgagctgtt caagaacgtg gccgagcggg gcagatggtc caagatgttc atgtccgcct
     2761 actccgcctt ccggaacaga agataccacg aggccttcgt gacctaccag ctgctggccg
     2821 agctgggcta cgaggtggcc cagagcaacg tggccttcat cctggaagag gaaaaggcca
     2881 ccggcatccc taaggacgag atccacaagc gggccttcac ccagtggcag agaagcgcca
     2941 cccagggcag caccagcagc agagtgaagc tgggcgacta ctactactat ggcctgggca
     3001 ccgacgtgtc ctaccagaag gccatccagc actaccggat cgcctccgac ctgcaccaca
     3061 acgcccaggc catgttcaac ctgggctaca tgcacgagca gggcctgggc ctgaagcggg
     3121 acctgtacct ggccaagcgg ttctacgaca tggccgccga agccagcatc gacgccagag
     3181 tggccgtgta tctggccctg atccggctga gcttctactt cgtgatggac ttcttcaacg
     3241 agagcagctt cttccagtac atcggcagca tgttcagcag cagaagctac gccgacgagc
     3301 acgaggtgga cagcgtgacc gacagcacca ccacccccac caccagctct caccccagcg
     3361 gccacctgat cagcacaggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     3421 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     3481 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     3541 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     3601 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     3661 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     3721 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     3781 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     3841 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     3901 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     3961 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     4021 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     4081 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     4141 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     4201 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     4261 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     4321 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     4381 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     4441 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     4501 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     4561 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     4621 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     4681 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     4741 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     4801 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     4861 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     4921 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     4981 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     5041 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     5101 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     5161 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     5221 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     5281 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     5341 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     5401 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     5461 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     5521 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     5581 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     5641 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     5701 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     5761 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     5821 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5881 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     5941 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     6001 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     6061 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     6121 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     6181 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     6241 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     6301 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     6361 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6421 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6781 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     6841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     7141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7741 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8401 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     8461 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8521 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8581 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     8641 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     8701 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     8761 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     8821 atcataatat gtacatttat attggctcat gtccaatatg accgc
//
