LOCUS       sequence-229159-       11799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_145420-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..11799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6963..6980
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    7032..7087
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7086..7105)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9323..9341)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9409..9513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9514..9582,9583..10374)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9732..9751)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10545..11133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11034..11053
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11287..11304
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11421..11442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11457..11462,11463..11480,11481..11487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11495..11511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11500..11522
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11519..11535
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11519..11535
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 acaatgccag catccctgcc accaccgaga gcaagaacct gcgggtgctg taccggttct
     1261 acaagcccga gaacctggac ttcgtgtacg tggccagcgt gaacagcatc cggcagttca
     1321 gcgtggacaa gctgcacctg gtggccgagc ggatcaccgg ccctcacaac ttcagcatct
     1381 actgcctgag caacggcgac agctgcgaga agtgcatgca cgtggacaga agcagcttcc
     1441 ccttcggcta cggctccgac ttctgcctgc cccaccccac caacaactac gtgaaggcct
     1501 ggggcaccag cggcttccct ggccccgaga tctacagctt caactacagc gagtgggacc
     1561 acgagagcca cttcgtgaac gacgaccgga tcaccttcga ggacagcctg tacgtgtgca
     1621 acaccgtgtt ccacggctac tgcgagcggc tgggcctgat caacgtgtcc caggaaagcc
     1681 cctggtccat cggcacccgg aaccccctgg tggacaagaa gaacaagaac ccccaggaaa
     1741 tccccgtcgt cggcagcctg ccctacaccc actctgtgct ggtcgtgggc agcgagtaca
     1801 tctacgtggg caccgagccc gacggcctgc aggaacacac cctgatcacc tttgaccccc
     1861 tgaccgtgcg gctgaagaac ttccacctgt gcagcaccca gcccagcttc gagagcagag
     1921 cccagctgag caccaacccc agcctgcgga tccagtacaa gagcgccttc cggtacagaa
     1981 gcctgcccaa caacctgaac ttccaggacc agctgttcag acccgcctac gtgtacttcg
     2041 tgctgcagca gcccgagagc agcagcttct tccggtggca gagccggatc gccagagtgt
     2101 gcgacggcga caagttcatc agcagctacg tggaactgaa tatcgcctgc ttcgagtgcg
     2161 tgttcgagga cgacgagaag atgttcaacg ccctgcactt cagcagccac ggcaccgtgg
     2221 gcaagcagct ggccagagag ctgctggaaa acttccagag ccagtacgac gacaacctgt
     2281 acagcaagat cgacatcacc accaccgacc acagcgaggt gctgatcgtg gccttcgcca
     2341 gccagcccat cgaccccctg tactactggt ccacccggaa cgtgtccccc gacatcgacc
     2401 tgcccaacgt gcagtacaac cccgacagca gctaccggca cggcagcatc ctgctgcctg
     2461 gcagcgccat cagcctgttc agcatgaccc agatcaacgc caagttcgac cgggtggtca
     2521 tgaactgcct ggacggcatc ggcatgcacg gccccagcca ctttctgcac agcggcagca
     2581 gcggcaaccc cagatgcgtg aaggacgact acagccggga cctgatccac gactgcccca
     2641 gcagccggca gctgaaccag ttcatcaccg gcaacgaccc cgacgacgac atctacagcc
     2701 ctgccctgct gtacttcaag agcaacgtga ccggcatggc catcaccacc gtgtctcagg
     2761 aattcaccgt cgccatcttc accaccgacg acggctacct gtataagtac gagctgctgg
     2821 gactgcggaa ggtgcacctc ctgggcagcc tgcagctggt gcccaacggc cagcctatca
     2881 ccagcctggc cctggaccac accggcacaa tcgcctttgc caccagcttc cagaaggtgt
     2941 tccggatcga gctgttcaac tgcagcgcct acctgacctg ccagagctgc ctgaaggccc
     3001 gggaccccta ctgcggctgg tgtatcgccg agggccggtg cctgctgttc caccagtgcc
     3061 ccctggtcat gagcagagtg aaaaccccca acgacctgaa gaagaagcgg agccccaaca
     3121 gattcgccgg ctccgagtac agcgccagcg accgggtgtt ctggctgcac tacaacacct
     3181 ccgtgaacaa gtgccccgtg atcagacagg tgtccccacc cggcatccag atcagcacca
     3241 cccccatggt cacctacgcc aaagagaaca gccagtacta ccagcagaac atcatcctga
     3301 gcctggacgc cttcctgagc cagaacctgc ctaacagcga cctgagcgag gaaagcttca
     3361 gccggaacca gccccaccag atcaacagct tcgtggactc cggcaacccc caccacccta
     3421 gcctggacag atccagcacc gccaccaaca gccagctgtt ctgcagcttc cgcgaggccc
     3481 ccaccagcgt gctgcagatg gaaagcctga tcagcggcac cctgagcaac ttcgacctga
     3541 acaagatcct gctgaacgtg accagaaccc ccctgctgac ccggaccgtg gccaagctgt
     3601 tctactccag cggcaagccc atcgaggccc actgcctgtc ccccgccatt gccagcagac
     3661 tgcctcagct ggaagatgac aaggccagcg tgcccctgct gctgtggctg gaaaaagtgc
     3721 ggaccaccga cgtggacccc gacaacgtgg gagccctggc ccctgccctg ttcgccgtgt
     3781 acaactgcag ccagctgaag gactgccagt cctgcacccg gtccagattc gtgtgcgcct
     3841 ggtgcctgtt tgaggaccag tgcgtgcccg tgttccccac cctgtccagc gacggccaga
     3901 tcaccaatac cgtgtgcaag gacagcagcc acctgtactc caagtcctac gagcagatca
     3961 accccccctt cgacatcatc ccccctggcc acgccgacga ctgccctagc ttcaagggca
     4021 gccccaagag aatcaccctg accagcggct ccagcctgac cgtgaacctg tatgtgtcca
     4081 atgtgcagaa acagcagatc gtgggcttca gctgcgccga gaactgcacc aaccagcacg
     4141 tgcgggccag cttcaacccc agcaacagca ccgtgttctg ccacatggaa gtgatgaacc
     4201 tgaccgagca cctgagcacc cggctgctga acaacaacga catccagcgg agccacgaca
     4261 ccgccgtgat ctgcgacctg gacctgtact ggcacggcct gaacagccac gacgccaagg
     4321 gccaccggat ggtcaacgtg gaccaggtgc aggtcgaggt gtacgcctgc gagtggctgg
     4381 ccgagtactg cgacaagtgc ctggacctgc cccccagatt cggctgctct tggtgcgtgt
     4441 ccgcccagga aaacatccac atcaacgact acgagaacaa gaatatcctg aacgagggca
     4501 agtgccggac ccagaacagc tgcgtgaccc ggaagtactt caacggctct atcgccgtgc
     4561 ggcagcacag catcagaccc ggcgacgtgt gccccgaccc cgagatcctg accctgagcc
     4621 ccaccaatgc caccctgacc ggccagccaa tcctgaccat caccggccgg aacctgggca
     4681 gaagcgcctc cgatatcgtc gaggtgttcc tggacctcca gagcaagatc cagtgcatcg
     4741 tgctgagcga gacataccag agaagccaca agttcatgtg caagctggtg gccgctcccc
     4801 acctgagcct gcctgtgtcc ggcaagctga aggtggtcat cagcaacaag agatacgagg
     4861 ccttcagccc tgtgttccac ttcgccgtgc cccatctgat ccacgccacc ccccagagag
     4921 gccctaaggc tggcggcacc agactgtacc tgagcggcac caacctgaac atcggccggc
     4981 agcgggccgt gtacctgtac ctgcctacca tctccggcac cagcgacgag agctaccagg
     5041 accggttcag cgagagcgac gtgcaggtca aatgcgagat cgagcaggaa agcgagacac
     5101 tgatcgtgtg cctgaccacc cctctgctga aagaaaccat gaacaccatg aagaacaaaa
     5161 agcagttcaa gcggcacagc ctgttcgtga acagctacta caacgacaac aacaagaccc
     5221 tcatcacccc cgagacaacc acaatcgccg aggacgagca ccacatcccc ctgagcctcg
     5281 tgatcatgca cgacatgacc ctgacctacc tgagccacag cttcagcttc atctacaccc
     5341 ccaacccctc catcctggaa gtgcagcggc agaacgtgct cgtgggcgga ggcaccacag
     5401 tccgggtgct gggcagcttc ctgttcgtgg tgcaggaccc ccggctggtg ttctacttct
     5461 ccggaagcga gtacagcatg agctgcctgg tgtcctccaa tggccagctg gactgtctga
     5521 gccccagcct gggcatgttc caggacaccc ccttcgtgaa ggacatgaac gacaagcaca
     5581 tctacaccag cgagaagctg gacaacctga tgcagagcag caccagcgtg tggaccaacg
     5641 gcaaagaaag cgtgaccagc tgcctgccca tcagccagcc cctggtgctg tcccagctga
     5701 agtccaacag agagttctac agcagcgcct tcaccagcca cccccccgac tggcccacag
     5761 acgtgcccta cggcttcatc atggacggcg tgcaccagct gagaatcttc ggactgatta
     5821 aggtgtacca ggaccccatc gtgatccctt tccccgacgg catccgggtg gaaaacctgg
     5881 acgacgatta cctgaccgac gatctgatga acgaccagca tgtcgagctg aacacctacc
     5941 agcggcagat cagcaaccag agcctggaac ccagcagcca gagcgtgtcc agcaccaact
     6001 cccacacact gagcaaccac atcgactaca gcaatgtgcg gcagctgatc cggatccacg
     6061 gccacttcga cgccctgatc aatgcccccg agctggccaa gtccgatgag ctgatcgtgc
     6121 ggatcggcga cagcctgatc tgcgaagtga actctatcat cctcaccgag atcagatgcg
     6181 atctggaccg gaagatgatc ctgaagcacc aggactaccc cgtcgaggtg cagttcggcc
     6241 ggttcctgat ctacagaccc ggcagcgtca gattcgtggc cctgagacag gccgccctgc
     6301 ggagccagat tggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     6361 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     6421 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     6481 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     6541 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     6601 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     6661 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     6721 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     6781 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     6841 ccaagatcca ggttttatcc aaagggttga attccggcag cctgcaccac atcctggacg
     6901 cccagaagat gctgtggaac cacagggaca ggaacctgcc tcctctggcc cctctgggcc
     6961 cccaccacca tcaccaccat tgagtctaga cacgtgatta attaaggatc ccccgacctc
     7021 gacctctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     7081 tctctcactc ggaaggacat atgggagggc aaatcatttg gtcgagatcc ctcggagatc
     7141 tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
     7201 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
     7261 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     7321 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     7381 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     7441 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     7501 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     7561 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     7621 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     7681 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     7741 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     7801 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     7861 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     7921 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     7981 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     8041 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     8101 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     8161 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     8221 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     8281 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     8341 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     8401 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     8461 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     8521 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     8581 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     8641 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     8701 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     8761 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     8821 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     8881 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     8941 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     9001 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     9061 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     9121 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     9181 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     9241 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgataagctg tcaaacatga
     9301 gaattttctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     9361 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     9421 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9481 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9541 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9601 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9661 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9721 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     9781 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9841 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9901 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9961 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    10021 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
    10081 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    10141 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    10201 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10261 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10321 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10381 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10441 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10501 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10561 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10621 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10681 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10741 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10801 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10861 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10921 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10981 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11041 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11101 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11161 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11221 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    11281 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    11341 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    11401 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    11461 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    11521 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacca attctcatgt
    11581 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
    11641 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
    11701 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
    11761 atatgtacat ttatattggc tcatgtccaa tatgaccgc
//
