LOCUS       sequence-22916-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V19 pCD-beta g FLT
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..87)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     5'UTR           complement(101..143)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     intron          179..275
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(314..643)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      328..463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     628..648
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ttcgttggac tcgtatacga ctcactatag ttctagaggc tcgagaggcc ttgaattcga
       61 gatcttatcg tcgtcatcct tgtaatccat ggtggatctg ccaaagttga gcgtttattc
      121 tgagcttctg caaaaagaac aagcaagctt agagcagaag taacacttcc gtacaggcct
      181 agaagtaaag gcaacatcca ctgaggagca gttctttgat ttgcaccacc accggatccg
      241 ggacctgaaa taaaagacaa aaagactaaa cttaccagtt aactttctgg tttttcagtt
      301 cctcgaggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
      361 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
      421 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
      481 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
      541 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
      601 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agccaagctt
      661 atcgatgata agctgtcaaa catgagaatt ggtcgactgg cgtaatagcg aagaggcccg
      721 caccgatcgc ccttcccaac agttgcgcac ctgantggcg agcgnccccc gntgnnnncc
      781 caaaccccgg gggggnntnn cngngannnn nnttnnnnnn cncccntttt tttttttttt
      841 ttttttttt
//
