LOCUS       sequence-229160-        8292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_149740-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3456..3473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3525..3580
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3579..3598)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5816..5834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5902..6006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6007..6075,6076..6867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6225..6244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7038..7626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7527..7546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7780..7797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7914..7935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7950..7955,7956..7973,7974..7980)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7988..8004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7993..8015
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8012..8028
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8012..8028
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 ttgagtgcgg caagcccgcc atctacctga acaacgagtt ctgggtcacc gacctgaccc
     1261 agggctgcct ggaagatgag ctgagcatcc tgaactactg caagaaggtg tacagcgagc
     1321 ggaacatcac cgccgtggtg tccgcccctc ccatcgacgt ggtgctgagc gattggtgcg
     1381 agttcgccca caagaaactg gccaagtgcc agagcatcgg cgacaagcgg cacgagatca
     1441 agcccttcat ctgcctggac aacatcccca tgaccaccct gttcaccccc gtgggctgcc
     1501 agctgtcctc cctggacaac gagcggagct acgtgtgcga gaactacaag ttctgggaga
     1561 gcagcaccag agaggcctgc ctgatccgga acatgcggtt ccagagctac ctgccctaca
     1621 agccctgcat cagcgagaag gacttccggt ccatgtactt cgccgctgcc aaggtcgtgt
     1681 gctgcaagaa cctggacagc gacttcacca actctgccgc cgtgatgaag cccctgaccg
     1741 accccggcaa caagaccagc agcatgagcc acaagcccag cagactgggc aacaagctga
     1801 ccaccaccga gaacggcatc tgggacagcg acaagaccgt gatcaactac ctgagcttca
     1861 ccaagcagaa cagcagcagc ggcctgctga tccccgagag agagcggtac agccaggcca
     1921 agctggccct gagcgacgac ctgcacaaga gagagaaggc cctggaacgg aagttcagca
     1981 gcgaggaaag cctgatcagc cccgaggact ggctgaacga gcccgtgaag tcccagctgg
     2041 aagaggacag cctggtgcag gaatttctga gcaagttcgt gaagctggaa aaccagagcg
     2101 agcaggacag aaagaccctg gaaaccatcc accaccagcg gatcgaggcc cagatgctgg
     2161 aacggcggaa cgccatgcag aacgcctggg agaaggccgt gaacatcgag aaccccaacc
     2221 accccgagct gagcccccct ctgctggcct acgccaacta cctgcggaac aacaagtacc
     2281 gggacctgga aagcaagagc aaggccgtgc tggtggccga cgtgaccctg cccgacatga
     2341 tcaagctgcc caccttccag gaacaggtca gcctgaaggc cgaggaaatc atcaccaagc
     2401 accggcacca cctggaaccc ctgtacagcg ataagctgac taccaacaag agcaacaagt
     2461 cccggatcaa gccccccaga cggcacaaga tgctgatcaa ccaccccttc ggcggaaaca
     2521 agaacaagaa cttcagcaac agccaccaga tgaagaccac caccatcatc cccaccgtgt
     2581 ccaacctggt ggatatcgac gtgaagtcct tcctgagcac cgtggactac agcagctccc
     2641 tgagcctgaa ccagaaccag cacacccccc acatcaacag cagcgagatg accgagaaca
     2701 agaccaccgt gcagttcggc agctaccaga acatcagcgt ggtcatgctg cactccgaca
     2761 aggtgctgaa caccagcaac agcagcaccg tgaacctgag cacaggcgcg ccgtcgacct
     2821 ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca ctcaaccttg
     2881 gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct tcttcccagt
     2941 cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct actagcaacc
     3001 ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag gtctcccttc
     3061 agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg tatcaggaag
     3121 tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc tgtgaggtga
     3181 tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag gaggccaggg
     3241 tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg tggcaatgtc
     3301 tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta tccaaagggt
     3361 tgaattccgg cagcctgcac cacatcctgg acgcccagaa gatgctgtgg aaccacaggg
     3421 acaggaacct gcctcctctg gcccctctgg gcccccacca ccatcaccac cattgagtct
     3481 agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa ggaaatttat
     3541 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3601 ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga tccccgcccc
     3661 ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat
     3721 cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg
     3781 ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca
     3841 tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca
     3901 caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg
     3961 tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct
     4021 gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc
     4081 cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact
     4141 aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta
     4201 gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt
     4261 acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga
     4321 agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg
     4381 tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa
     4441 acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt
     4501 gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa
     4561 tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga
     4621 ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat
     4681 gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt
     4741 acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt
     4801 tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag
     4861 acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg
     4921 ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg
     4981 ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc
     5041 tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat
     5101 tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc
     5161 gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat
     5221 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     5281 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     5341 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     5401 agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt
     5461 agtatatgct atcctaattt atatctgggt agcatatact acccaaatat ctggatagca
     5521 tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca
     5581 taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta
     5641 tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca
     5701 tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca
     5761 tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc
     5821 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5881 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5941 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6001 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6061 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6121 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6181 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6241 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6301 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6361 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6421 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6481 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6541 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6601 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6661 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6721 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6781 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6841 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6901 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7741 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7801 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7861 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7921 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7981 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     8041 aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg cagatccggg
     8101 caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc tatacattga
     8161 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
     8221 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
     8281 caatatgacc gc
//
