LOCUS       sequence-229161-        9903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_155810-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5067..5084
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5136..5191
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5190..5209)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7427..7445)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7513..7617
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7618..7686,7687..8478)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7836..7855)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8649..9237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9138..9157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9391..9408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9525..9546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9561..9566,9567..9584,9585..9591)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9599..9615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9604..9626
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9623..9639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9623..9639
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 tgatccagaa catcaacagc cccatcgacc acgagtacta catcgaggaa gagatcccca
     1261 tcggcaccat catcggcaat atcatggacg acctgatcat gaagtacatc gacttcggcc
     1321 tgaccaagtt cctgcacaag ctgaactccc ccatcaacga caacgaccac agctacagcc
     1381 tgatgatcat gaacttcgcc gacccctacg tgaacctgtt cgacatcaag ctggacggcc
     1441 ggatcatcct gagcagacgg ctggaccggg accagatctg ccccaagcag cagcacaccg
     1501 acctgcccct ggtcaacatc gacagcgccc agttcagcag cgtgtacacc agcacccacc
     1561 ggcagaacat cctgcagacc gagaagatca gctgcagcat cattctgcag atcggcctgt
     1621 tcatccacca gaagaacggc atcatcaaca aagtgaacac cgaggccaac aacaagaagc
     1681 agatccggct gttccacatc cggatcaaca tccaggacat caatgacaac gccccctact
     1741 ggcagggcca tctgcagcgg ttccggatca ccttcctgga cggcgacccc gtgggcagca
     1801 gaagaaacat cccccctgcc atggacaacg acgagggcat caacggcctg atcacctacg
     1861 agctgagccc cgtgaacagc ttcgacaaga gccacatccc cttcagcctg ctggaagata
     1921 gcatcgacgg cctgcacctg tacgccacca agcagatcga cagagaggaa cagagccagt
     1981 acaagctggt gctgaaggcc accgacggca gctactccct gagcagctct accagcagcg
     2041 ccctgagcaa cgagagcacc ttcaagcggt tcacctccac cctgtacgtg gacatcttca
     2101 tcgaggacgt gaacgacaac accccccagt tcacccagcc catcttcacc accagcatgc
     2161 ctatcgccga gacaaccccc gtgggcgcca ccgtgctgct gctgaacgcc acagacctgg
     2221 acagcggcat caatggcatc ttccacttca gcttcagcaa ggaccactac tggcagcccg
     2281 ccgagaagat tgcccggcac tacttcgaca tccggcccaa cggccagatc attgtgaagc
     2341 agcccctgaa tgtggacaag ctggaccaga tcaatagcca catcaaccac aacaacaaca
     2401 aaatcgtgct gcccaacatc agaggcgcct accagctgaa ggacgacatc gacgagaata
     2461 tcaacaaggg cgtgaacgtg cagttccggt tccgggccgt ggtggaagat gaggccaccc
     2521 ggccctacac cagatccagc gaggccaccg tgatcatcct ggtcaccgac gagaacgacg
     2581 agtcccccgt gatctccctg ctgcccaatc ccgagctgat cagcagcaac gccctggacg
     2641 ccgtgaccac cagcaacagc ttcagctact tctgggtgtt cgagaaccag cccgtgggca
     2701 ccacagtggc caccgtgcag gcctacgacc ccgactttgg cggccaggac caggtggaat
     2761 gcaacctgaa ggacagcaac ttcagcctga tccggctgac cagccagacc cagcccgagt
     2821 tcgaccggaa caccgaagtg aacctgctga ccgagtacca cctgatcacc gcccggatcc
     2881 tggaccgcga gagcaccagc caccaggtgg tcaccatcgt gtgcagcgac atggtcggac
     2941 actacaccga gcggaacacc accgtgcgca tcctggacga gaatgacaac cggcccaagt
     3001 tcagccacga gatcctgtat ctgcaggtcg acgaaaacgc cccttacggc acccaggtca
     3061 tccagcagag ccagaaagtg aaccggggcc acttcaacaa cgtgcaccag accggcagcc
     3121 tgcagggcct gctggccagc gacctggata tcggaatcaa cagccagatg acattcttcc
     3181 tgttcgagga cggcatcaac agcaccaagt tcaagatcga cagccacaca ggcatcatca
     3241 ccaccatgga tatcttcgac cgggaagtga aagagaagta caccctgatc gccctggccg
     3301 tggaccacgg caatcctcca ctgaccggca ccgccacagt gcagatcatc atcaacgacc
     3361 tgaacgacaa tggccccgtg ttcagcacca acaactacac attccatctg caggaaaacc
     3421 aggcccggtc caccatcgtg ggccaggtca acgccaccga cgtggactct agcgcctacg
     3481 gccccctgga atactccttc gccaacgacc ccgatagcgt gaacttcacc atcgacaaga
     3541 tcaccggcat catccggaac cggcagagcc tggacagaga agagaagtcc cagtacatct
     3601 tccgggtgct ggtcaaggac accaagatcc ggcccagcga gttcaacagc atcagcatca
     3661 acaagctcaa cagcaagagc atccagtaca ccggcaccag caccgtgacc gtgatcgtgg
     3721 acgatgtgaa cgataactgg cctgtgttcg tgtcccccaa cagcaccgcc aacaccctgg
     3781 ccattgccct ggaccagaag atcacaggct acaagctggc ctacatccag gccgaggaca
     3841 aggatgaggg caacaatggc accatcacct acaacatcct gaccggcaac aactacggcc
     3901 tgttcggcct ggacagcacc agcggcctgc tgtacctgag caaccccttc agcgacaaca
     3961 acaagctgag cgaccacaac aatgagctgt acgacagcag caacaccagc aacctgccca
     4021 ccaacgacga gttcaatggc ctgctgcaca agagcagcat ccacatcctg acactggaag
     4081 cctgcgacca gggccaggac cccaagccca gatgcaccct gtacaacaac ctgaagatca
     4141 tgattcagaa caacaaagag aaggacgtcc acaccctgga aagcaagcag ttccagtaca
     4201 actcccccaa ccagttcatc aacgcccctg gcctgaccag cgccgagaca gtgctgagca
     4261 gcgccttcaa gcagaaccac cccaaccaca tccagaacaa cttcaactcc atccaggaat
     4321 tcaacaacga caacctgtac cagaaggcca caggcctgac ctacggcaac ctgctgatga
     4381 gcaagcagaa tcacaagctg tccagctaca ccacaggcgc gccgtcgacc tccatcacgg
     4441 cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt ggagaggaaa
     4501 gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag tcctggatca
     4561 ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac cccaagttcc
     4621 agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt cagtttgctg
     4681 gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa gtgaacctgg
     4741 tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg atgggaccca
     4801 cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg gtctccaggc
     4861 aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt ctactgagtg
     4921 aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg ttgaattccg
     4981 gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg gacaggaacc
     5041 tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc tagacacgtg
     5101 attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta ttttcattgc
     5161 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     5221 tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc cggacgaact
     5281 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     5341 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     5401 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     5461 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     5521 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     5581 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     5641 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     5701 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     5761 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     5821 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     5881 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     5941 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     6001 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     6061 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     6121 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     6181 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     6241 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     6301 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     6361 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     6421 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     6481 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     6541 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     6601 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     6661 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     6721 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     6781 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     6841 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     6901 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     6961 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     7021 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     7081 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     7141 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     7201 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     7261 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     7321 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
     7381 ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct cgtgatacgc
     7441 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7501 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7561 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7621 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7681 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7741 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7801 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7861 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7921 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7981 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8041 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8101 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8161 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8221 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8281 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8341 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8401 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8461 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8521 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8581 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8641 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8701 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8761 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8821 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8881 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8941 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9001 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9061 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9121 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9181 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9361 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9541 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9601 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag
     9661 ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg gcaacgttgt
     9721 tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg aatcaatatt
     9781 ggcaattagc catattagtc attggttata tagcataaat caatattggc tattggccat
     9841 tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac
     9901 cgc
//
